慢性萎缩性胃炎治疗组方逆萎康优化研究

RESEARCH ON OPTIMIZATION OF THE PRESCRIPTION NIWEIKANG FOR THE TREATMENT OF CHRONIC ATROPHIC GASTRITIS

目的对治疗慢性萎缩性胃炎的逆萎康组方进行优化,以减少其不良反应。方法健康昆明种小鼠10只,以水杨酸钠乙醇混合溶液灌胃10 d制备急性胃炎小鼠模型,处死小鼠观察胃黏膜病理组织学改变。70只健康昆明种小鼠随机分为7组,按上述方法先制备急性胃炎小鼠模型,再分别灌胃雪胆煎煮液(雪胆组)、黄芪煎煮液(黄芪组)、老龙皮煎煮液(老龙皮组)、朱砂七煎煮液(朱砂七组)、半枝莲煎煮液(半枝莲组)、海螵蛸煎煮液(海螵蛸组)、生理盐水(生理盐水组),每天记录所有小鼠食用高岭土和鼠粮的质量及小鼠体质量,并计算高岭土/鼠粮比值,根据其比值筛选致呕成分。50只健康昆明种小鼠鼠随机分为5组,先制备急性胃炎小鼠模型,再分别灌胃原逆萎康组方煎煮液(A组)、原逆萎康组方朱砂七减半煎煮液(B组)、原逆萎康组方加生姜煎煮液(C组)、原逆萎康组方加陈皮煎煮液(D组)、原逆萎康组方加半夏煎煮液(E组),每天记录所有小鼠食用高岭土和鼠粮的质量及小鼠体质量,并计算高岭土/鼠粮比值,根据其比值筛选最优组方。结果小鼠急性胃炎模型建立成功。单因素方差分析结果显示,致呕成分筛选实验中,7组小鼠食用高岭土/鼠粮的比值差异具有统计学意义(F=13.92,P<0.05);与生理盐水组比较,仅朱砂七组小鼠该比值差异具有显著性(P<0.05),其他组小鼠该比值差异无显著性(P>0.05)。最优组方筛选实验中,5组小鼠食用高岭土/鼠粮的比值差异具有统计学意义(F=3.63,P<0.05);与A组比较,仅D组小鼠该比值差异有显著性(P<0.05),其他组小鼠该比值差异无显著性(P>0.05)。结论原中药逆萎康组方中朱砂七是导致小鼠恶心的主要成分,在原组方中加入陈皮可作为慢性萎缩性胃炎治疗的新组方。

Objective To optimize the prescription Niweikang for the treatment of chronic atrophic gastritis,and to reduce its adverse effects.Methods A total of 10 healthy Kunming mice were given a mixed solution of sodium salicylate and etha-nol by gavage for 10 days to establish a mouse model of acute gastritis,and then the mice were sacrificed to observe the histopathological changes of stomach.A total of 70 healthy Kunming mice were used to establish a mouse model of acute gastritis and were then given Hemsleya root decoction(Hemsleya group),Astragalus membranaceus decoction(Astragalus membranaceus group),Lobaria kurokavae Yoshim decoction(Lobaria kurokavae Yoshim group),Polygonum cillinerve decoction(Polygonum cillinerve group),Scutellaria barbata decoction(Scutellaria barbata group),cuttlebone decoction(cuttlebone group),or normal saline(normal saline group)by gavage,the amounts of kaolin and rat diet and the body weight of mice were recorded daily to calculate kaolin/rat diet ratio,and emetic ingredients were screened out according to this ratio.A total of 50 healthy Kunming mice were used to establish a mouse model of acute gastritis and were then given original Niweikang decoction(group A),Niweikang+Polygonum cillinerve decoction reduced by half(group B),Niweikang+ginger decoction(group C),Niweikang+tangerine peel decoction(group D),or Niweikang+Pinellia ternata(group E),the amounts of kaolin and rat diet and the body weight of mice were recorded daily to calculate kaolin/rat diet ratio,and the optimal prescription was screened out based on this ratio.Results The mouse model of acute gastritis was established successfully.The one-way analysis of variance showed that in the experiment for the screening of emetic ingredients,there was a significant difference in kaolin/rat diet ratio between the 7 groups(F=13.92,P<0.05),there was a significant difference in this ratio between the Polygonum cillinerve group and the normal saline group(P<0.05),while there was no significant difference in this ratio between the normal saline group and the other 5 groups(P>0.05).In the experiment for the screening for the optimal prescription,there was a significant difference in kaolin/rat diet ratio between the 5 groups(F=3.63,P<0.05);there was a significant difference in this ratio between group A and group D(P<0.05),while there was no significant difference in this ratio between group A and the other 3 groups(P>0.05).Conclusion Polygonum cillinerve is the main component causing nausea in the original tradition Chinese medicine Niweikang prescription,and the original Niweikang prescription with the addition of tangerine peel can be used as a new prescription for the treatment of chronic atrophic gastritis.