长链非编码RNA TTTY15/MIF-AS1在HPV感染宫颈癌组织中的表达及其与细胞自噬的关系

Expression of long non-coding RNA TTTY15/MIF-AS1 in cervical cancer tissues and its relationship with autophagy in HPV infection

目的探讨长链非编码RNA(LncRNA)TTTY15/MIF-AS1在人乳头瘤病毒(HPV)感染宫颈癌组织中的表达及其与细胞自噬的关系。方法选择2018年12月-2021年12月天津市第五中心医院妇产科收治的宫颈癌患者82例为宫颈癌组,选择同期医院进行宫颈检查的宫颈低级别上皮内病变(LSIL)和宫颈高级别上皮内病变(HSIL)患者各50例分别作为LSIL组和HSIL组。采用免疫组化法检测研究对象宫颈组织中LncRNA TTTY15、LncRNA MIF-AS1相对表达量。采用实时荧光定量聚合酶链式反应(RT-PCR)扩增自噬通路基因自噬相关基因-12(ATG12)、BECN1和微管相关蛋白1A/1B轻链3B(MAP1LC3B),均以2法计算其相对表达量。结果宫颈癌组、HSIL组和LSIL组LncRNA TTTY15、LncRNA MIF-AS1、ATG12、BECN1和MAP1LC3B基因相对表达量差异有统计学意义(P<0.05),其中宫颈癌组高于HSIL组和LSIL组,HSIL组高于LSIL组(P<0.05);不同淋巴结转移、肿瘤大小和分化状态宫颈癌患者LncRNA TTTY15相对表达量差异有统计学意义(P<0.05);不同淋巴结转移和分化状态宫颈癌患者LncRNA MIF-AS1相对表达量比较差异有统计学意义(P<0.05);宫颈癌患者宫颈组织中LncRNA TTTY15、LncRNA MIF-AS1相对表达与自噬通路基因ATG12、BECN1和MAP1LC3B基因相对表达量均成正相关(P均<0.05)。结论LncRNA TTTY15、LncRNA MIF-AS1参与高危型HPV感染宫颈癌病理进展过程,其机制可能与自噬通路有关。

OBJECTIVE To explore the expression of long non-coding RNA(LncRNA)TTTY15/MIF-AS1 in cervical cancer(CC)tissues and its relationship with autophagy in human papilloma virus(HPV)infection.METHODS A total of 82 patients with CC admitted to Obstetrics and Gynecology Department of Tianjin Fifth Central Hospital were enrolled as CC group between Dec.2018 and Dec.2021,while 50 patients with low-grade squamous intraepithelial lesions(LSIL)and 50 patients with high-grade squamous intraepithelial lesions(HSIL)who underwent cervical examinations during the same period were enrolled as LSIL group and HSIL group,respectively.The relative expression levels of LncRNA TTTY15 and LncRNA MIF-AS1 in cervical tissues were detected by immunohistochemistry.The autophagy pathway genes[autophagy-related gene-12(ATG12),BECN1,microtubule-associated protein 1 light chain 3 beta(MAP1 LC3 B)]were amplified by real-time quantitative polymerase chain reaction(RT-PCR),and their relative expression levels were calculated by 2method.RESULTS There were significant differences in relative expression levels of LncRNA TTTY15,LncRNA MIF-AS1,ATG12,BECN1 and MAP1 LC3 B among CC group,HSIL group and LSIL group(P<0.05),which were the highest in CC group,followed by HSIL group and LSIL group(P<0.05).There were significant differences relative expression level of LncRNA TTTY15 among CC patients with different lymph node metastasis,tumor size and differentiation status(P<0.05).There were significant differences relative expression level of LncRNA MIF-AS1 in CC patients with different lymph node metastasis and differentiation status(P<0.05).The relative expression levels of LncRNA TTTY15 and LncRNA MIF-AS1 in cervical tissues were positively correlated with relative expressions of autophagy pathway genes(ATG12,BECN1,MAP1 LC3 B)(P<0.05).CONCLUSION LncRNA TTTY15 and LncRNA MIF-AS1 are involved in the pathological progression of cervical cancer in high-risk HPV infection,and the mechanism may be related to autophagy pathway.