响应面法优化葛根蛋白酶解工艺及其体外抗氧化特性分析

Optimization of Enzymatic Hydrolysis Process of Pueraria Protein by Response Surface Methodology and Its Antioxidant Properties in Vitro

目的:优化葛根蛋白酶解工艺并研究其抗氧化特性。方法:以DPPH自由基清除能力、水解度(DH)为评价指标,结合SDS-PAGE电泳结果,筛选最佳水解蛋白酶;在单因素实验基础上,利用Box-Behnken响应面法优化葛根蛋白酶解工艺,并对最佳葛根蛋白酶解物进行抗氧化特性研究。结果:葛根蛋白酶解最佳工艺条件为:酶解温度55℃、pH9、酶底比2%,该条件下制备的葛根蛋白酶解物清除DPPH自由基、ABTS+自由基、OH自由基的IC_(50)值分别为0.15、0.38、1.41 mg/mL,还原能力为0.553。结论:该条件下制备的葛根蛋白酶解物具有较好的抗氧化特性。

Objective:To optimize the enzymatic hydrolysis process of Pueraria protein and study its antioxidant properties.Methods:DPPH radical scavenging ability and DH were used as the index to screen the best hydrolytic protease.Base on the single factor experiments,the Box-Behnken response surface design was used to optimize the enzymatic hydrolysis process of Pueraria protein,and the antioxidant properties of the optimum hydrolysate in vitro was analyzed.Results:The optimal hydrolysis conditions for alkaline protease were as follows:Temperature 55℃,pH9 and enzyme substrate ratio 2%.Under these conditions,the IC_(50) of DPPH·,ABTS^(+)·,and·OH radicals were 0.15,0.38,1.41 mg/mL and a reduction capacity of 0.553.Conclusion:Under these conditions,Pueraria protein hydrolysate had remarkable antioxidant properties.