摘要
目的:优化葛根蛋白酶解工艺并研究其抗氧化特性。方法:以DPPH自由基清除能力、水解度(DH)为评价指标,结合SDS-PAGE电泳结果,筛选最佳水解蛋白酶;在单因素实验基础上,利用Box-Behnken响应面法优化葛根蛋白酶解工艺,并对最佳葛根蛋白酶解物进行抗氧化特性研究。结果:葛根蛋白酶解最佳工艺条件为:酶解温度55℃、pH9、酶底比2%,该条件下制备的葛根蛋白酶解物清除DPPH自由基、ABTS+自由基、OH自由基的IC_(50)值分别为0.15、0.38、1.41 mg/mL,还原能力为0.553。结论:该条件下制备的葛根蛋白酶解物具有较好的抗氧化特性。
Objective:To optimize the enzymatic hydrolysis process of Pueraria protein and study its antioxidant properties.Methods:DPPH radical scavenging ability and DH were used as the index to screen the best hydrolytic protease.Base on the single factor experiments,the Box-Behnken response surface design was used to optimize the enzymatic hydrolysis process of Pueraria protein,and the antioxidant properties of the optimum hydrolysate in vitro was analyzed.Results:The optimal hydrolysis conditions for alkaline protease were as follows:Temperature 55℃,pH9 and enzyme substrate ratio 2%.Under these conditions,the IC_(50) of DPPH·,ABTS^(+)·,and·OH radicals were 0.15,0.38,1.41 mg/mL and a reduction capacity of 0.553.Conclusion:Under these conditions,Pueraria protein hydrolysate had remarkable antioxidant properties.
作者
庞会娜
董红影
肖凤琴
张红印
韩荣欣
王海东
严铭铭
邵帅
PANG Huina;DONG Hongying;XIAO Fengqin;ZHANG Hongyin;HAN Rongxin;WANG Haidong;YAN Mingming;SHAO Shuai(Changchun University of Traditional Chinese Medicine,Changchun 130117,China;Jilin Province Traditional Chinese Medicine Health Food Science and Technology Innovation Center,Changchun 130117,China)
出处
《食品工业科技》
CAS
北大核心
2022年第24期197-204,共8页
Science and Technology of Food Industry
基金
吉林省科技发展计划项目(20180623041TC)
吉林省教育厅科学技术研究项目(JJKH20210970KJ)
长春中医药大学2021年大学生创新创业训练计划项目。
关键词
葛根蛋白
Box-Behnken
响应面法
酶解工艺
抗氧化特性
Pueraria protein
Box-Behnken response surface methodology
enzymatic hydrolysis process
antioxidant properties