艾司奥美拉唑抑制乳腺癌细胞增殖及化疗增敏效果的研究

Research of esomeprazole on inhibiting proliferation and chemosensitization of breast cancer cells

目的研究艾司奥美拉唑对乳腺癌细胞的增殖抑制效应以及化疗增敏效果。方法常规方法培养人MBA-MD-231、MCF-7乳腺癌细胞系和人Huh7肝癌细胞系,用不同浓度的艾司奥美拉唑处理细胞,采用CCK8试剂盒检测刺激后不同肿瘤细胞的增殖情况;用不同浓度的艾司奥美拉唑处理细胞,利用流式细胞仪分析艾司奥美拉唑对不同细胞的细胞周期的影响;用不同浓度的紫杉醇、表柔比星联合艾司奥美拉唑处理细胞,采用CCK8试剂盒检测刺激后不同肿瘤细胞的增殖情况。计量资料以均数±标准差(±s)表示,多组间比较采用方差分析。结果CCK8试剂盒检测结果显示,艾司奥美拉唑可以抑制MBA-MD-231细胞、MCF-7细胞和Huh7细胞增殖,而且抑制作用呈剂量依赖性。流式细胞仪检测结果显示,艾司奥美拉唑处理不同细胞,G_(0)/G_(1)期细胞均显著增加。艾司奥美拉唑可增强化疗药物紫杉醇及表柔比星对MBA-MD-231细胞、MCF-7细胞的增殖抑制效果,提高化疗敏感性。结论艾司奥美拉唑将乳腺癌细胞MBA-MD-231、MCF-7和肝癌细胞Huh7阻滞于G_(0)/G_(1)期,从而抑制细胞增殖。艾司奥美拉唑可以增强化疗药物对MBA-MD-231和MCF-7细胞的增殖抑制作用。

Objective To investigate the inhibitory effect of esomeprazole on proliferation and chemosensitizing effect of breast cancer cells.Methods Human MBA-MD-231,MCF-7 breast cancer cell line and human Huh7 liver cancer cell line were cultured by conventional methods;cells were treated with different concentrations of esomeprazole,and CCK8 kit was used to detect the proliferation of different tumor cells after stimulation.Cells were treated with different concentrations of esomeprazole,and the effects of esomeprazole on cell cycle of different cells were analyzed by flow cytometry.Cells were treated with different concentrations of paclitaxel and epirubicin combined with esomeprazole,and CCK8 kit was used to detect the proliferation of different tumor cells after stimulation.Measurement data were expressed as mean±standard deviation(±s),and analysis of variance was used for comparison among multiple groups.Results CCK8 results showed that esomeprazole could inhibit the proliferation of MBA-MD-231 cells,MCF-7 cells and Huh7 cells in a dose-dependent manner.Flow cytometry results showed that cells in G_(0)/G_(1)phase were significantly increased by esomeprazole treatment.Esomeprazole can enhance the inhibitory effect of paclitaxel and epirubicin on the proliferation of MBA-MD-231 cells and MCF-7 cells,and improve the chemosensitivity.Conclusion Esomeprazole blocks breast cancer cell MBA-MD-231,MCF-7 and liver cancer cell Huh7 in G_(0)/G_(1)phase,thereby inhibiting cell proliferation.Esomeprazole can enhance the inhibitory effect of chemotherapeutic drugs on the proliferation of MBA-MD-231 and MCF-7 cells.