TYK2、STAT3与无功能性垂体腺瘤侵袭性相关性研究

Correlation between the expression of TYK2 and STAT3 in non-functioning pituitary adenomas and invasiveness

目的研究酪氨酸激酶2(TYK2)和信号转导因子和转录激活因子3(STAT3)在无功能性垂体腺瘤(NFPA)的表达与侵袭性的相关性。方法收集新疆维吾尔自治区人民医院神经外科自2020年6月至2021年5月收治的24例NFPA患者的临床资料和手术标本,根据术前核磁检查和术中所见情况分为侵袭性组和非侵袭性组,每组12例。用qRT-PCR、Western blot及免疫组化等方法检测2组患者NFPA组织中的TYK2和STAT3表达量,分析其表达量与NFPA侵袭性的相关性。结果qRT-PCR、Western blot及免疫组化结果均显示,与非侵袭组相比,侵袭组NFPA中TYK2低表达、STAT3高表达,差异均有统计学意义(P<0.05)。结论TYK2在侵袭性NFPA中表达下调,其低表达可能与NFPA侵袭性相关;STAT3在侵袭性NFPA中表达上调,其高表达可能与NFPA侵袭性相关。

Objective To investigate the correlation between the expression of tyrosine kinase 2(TYK2)and signal transducers and activators of transcription 3(STAT3)in non-functioning pituitary adenoma(NFPA)and invasiveness.Methods Clinical data and surgical specimens of 24 patients with NFPA treated in our hospital from June 2020 to May 2021 were collected and divided into invasive and non-invasive NFPA groups based on their pre-operative imaging and intra-operative information,comprising 12 patients for each group.TYK2 and STAT3 expressions in the NFPA tissues of the two groups were measured by quantitative real-time polymerase chain reaction(qRT-PCR),immunohistochemistry(IHC)and Western blotting,and the correlation among their expressions in NFPA and invasiveness was analysed.Results The results of qRT-PCR,Western blot and immunohistochemistry showed that low TYK2 and high STAT3 expressed in the NFPA of the invasive group compared to the non-invasive group(P<0.05).Conclusion The expression of TYK2 in invasive NFPA is down-regulated and its low expression may be associated with NFPA invasiveness;The expression of STAT3 in invasive NFPA is up-regulated and its high expression may be associated with NFPA invasiveness.