基于生物信息学对直肠癌中m6A调节因子介导相关修饰模式的分析

Analysis of m6A regulatory factor mediated modification patterns in rectal cancer based on Bioinformatics

目的探索N6-甲基腺嘌呤(m6A)修饰在肿瘤形成及与肿瘤微环境(TME)形成中的关系.方法基于23个m6A调节因子对288例直肠癌标本的m6A修饰模式进行综合评估,以确定直肠癌中m6A修饰模式和m6A相关基因特征,并评估基于PCA算法的m6A评分方案与免疫治疗反应之间的相关性.结果本研究分析了23个m6A调节因子在直肠癌中的表达和突变情况,及其与患者预后的关系,进一步在直肠癌样本中确定了三种不同的m6A修饰模式,分析其与不同的临床特征有关,并且证明m6A评分可以预测患者对抗PD-1/PD-L1免疫治疗的临床反应.结论m6A修饰异常与肿瘤的发生发展密切相关,并且在TME多样性和复杂性的形成中起着重要作用,评估肿瘤中的m6A修饰模式将有助于指导更有效的免疫治疗策略.

Objective To explore the relationship between m6A modification and tumor microenvironment(TME).Methods The m6A modification pattern of 288 rectal cancer specimens was comprehensively evaluated based on 23 m6A regulators(RC)to determine the m6A modification pattern and m6A related gene characteristics in RC,and to evaluate the correlation between m6A scoring scheme based on PCA algorithm and immunotherapy response.Results This study analyzed the expression and mutation of 23 m6A regulatory factors in RC and their relationship with the prognosis of patient.Further,three different m6A modification modes were identified in RC samples,which were related to different clinical characteristics.It was proved that m6A score could predict the clinical response of patients to PD-1/PD-L1 immunotherapy.Conclusions This study showed that the abnormal m6A modification was closely related to the occurrence and development of tumors,and played an important role in the formation of diversity and complexity of TME.Evaluating the m6A modification mode in tumors will help to guide more effective immunotherapy strategies.