HPLC同时测定姜黄醒酒饮料中3种姜黄素含量

Simultaneous Determination of Three Curcumins in Turmeric Sobering Beverage by HPLC

目的 建立高效液相色谱(HPLC)同时测定姜黄醒酒饮料中姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素含量的方法。方法 应用HPLC,采用Agilent ZORBAX SB-C_(18)(4.6 mm×150 mm,5μm),连接C_(18)保护柱(4.0 mm×3.0 mm,5μm),梯度洗脱,以乙腈-0.04%甲酸水溶液为流动相,流速为1.0 ml/min,柱温:25℃。结果 姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素浓度分别在1.95~250μg/ml,0.98~125μg/ml,0.98~125μg/ml范围内与峰面积线性关系良好(R^(2)=1,n=8);检测限(LOD)分别为0.49,0.24,0.24μg/ml,定量限(LOQ)分别为0.98,0.49,0.49μg/ml;平均回收率为92.67%~106.77%。结论 该方法具有灵敏度高,分析速度快,准确性高的优点,可用于不同品牌姜黄醒酒饮料中3种姜黄素类成分的含量检测。

Objective To establish an HPLC method for determination of curcumin,demethoxycurcumin and bisdemethoxycurcumin in Turmeric Sobering Beverage.Methods The samples were determined on an Agilent ZORBAX SB-C_(18)(4.6 mm×150 mm,5μm) column connected with C_(18)protection column (4.0 mm×3.0 mm,5μm),using acetonitrile-0.04%formic acid at a?ow rate of 1.0 ml/min as the mobile phase of gradient elution.The detection wavelength was 425 nm.The column temperature was controlled at 25℃.Results The contents of curcumin,demethoxycurcumin and bisdemethoxycurcumin had good linear relationships with the peak areas in the range of1.95-250,0.98-125 and 0.98-125μg/ml,respectively.The limits of quantitation (LOQ) were 0.49,0.24,0.24μg/ml,respectively;the limits of detection (LOD) were 0.98,0.49,0.49μg/ml,respectively.The average recoveries were92.67%-106.77%.Conclusion The method has the advantages of high sensitivity,rapid analysis and high accuracy,and can be used for the determination of three curcumins in Turmeric Sobering Beverage.