摘要
目的采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法(UHPLC-Q Exactive Focus MS/MS)对高粱泡叶的化学成分进行快速识别和鉴定。方法选择高粱泡叶的80%乙醇提取液作为供试品溶液,采用ACE Ultracore2.5 SuperC18(100 mm×2.1 mm,0.5μm)色谱柱(2.1 mm×150 mm,2.6μm),流动相乙腈(C)-0.1%甲酸水溶液(B)梯度洗脱,流速0.3 mL/min,柱温40℃;质谱分析采用加热电喷雾离子源(HESI),正、负离子模式扫描,扫描范围100~1500 m/z。结果共鉴定出了61个成分,包括游离氨基酸、酚酸类、黄酮类、有机酸类、糖苷类及其他类。结论该方法可系统、准确、快速地对高粱泡叶中化学成分进行定性分析,可为高粱泡叶药效物质研究及质量评价提供重要依据。
Objective To rapidly identify the chemical constituents of Rubus lambertianus Ser leaves by ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry(UHPLC-Q Exactive Focus MS/MS).Methods The 80%ethanol extract of Rubus lambertianus Ser leaves was selected as the test solution.The ACE Ultracore2.5 SuperC18(100 mm×2.1 mm,0.5μm)column(2.1 mm×150 mm,2.6μm)was used.The mobile phase was acetonitrile(C)-0.1%formic acid aqueous solution(B)gradient elution,the flow rate was 0.3 mL/min,and the column temperature was 40℃.Mass spectrometry analysis was performed using a heated electrospray ionization(HESI)source with positive and negative ion mode scanning in the range of 100~1500 m/z.Results A total of 61 components were identified,including free amino acids,phenolic acids,flavonoids,organic acids,glycosides and others.Conclusion:This method can systematically,accurately and quickly analyze the chemical components in sorghum leaves,which can provide an important basis for the study and quality evaluation of Rubus lambertianus Ser leaves.
作者
李旭东
吴静澜
田孟斌
聂琴
LI Xudong;WU Jinglan;TIAN Mengbin;NIE Qin(School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;National Engineering Technology Research Center for Miao Medicine,Guiyang 550025,China;Guizhou Engineering Technology Research Center for Processing and Preparation of Traditional Chinese Medicine,Guiyang 550025,China)
出处
《湖北民族大学学报(医学版)》
2022年第4期18-22,共5页
Journal of Hubei Minzu University(Medical Edition)
基金
贵州省中医药管理局中医药、民族医药科学技术课题(QZYY-2022-011)。