供体来源细胞游离DNA在肾移植中的研究

Research on donor-derived cell-free DNA in kidney transplantation

肾移植是终末期肾病有效治疗手段。移植物损伤是影响移植物存活的主要因素。常用方法如监测血清肌酐、尿蛋白及移植肾活检等,存在迟滞性,不确定性及有创性。供体来源细胞游离DNA(donor-derived cellfree DNA,dd-cfDNA)是一种源于供体的碎片化、可降解DNA片段。现有研究发现,dd-cfDNA在移植肾损伤监测、移植物排斥鉴别、免疫抑制方案调整等方面发挥重要作用,是一种无创、可重复且灵敏度高的新型生物标志物。本文就近年来dd-cfDNA在肾移植的研究进展做一综述。

Kidney transplantation is an effective treatment for end-stage renal disease.Allograft injury is a major influential factor of allograft survival.Commonly used surveillance methods,such as plasma creatinine,proteinuria,and kidney biopsy,were found to be hysteresis,uncertainty and invasiveness.Donor-derived cell-free DNA(dd-cfDNA)is a fragmented and degradable DNA fragment derived from transplanted organs.Sudies have shown that dd-cfDNA plays a pivotal role in the monitoring of allograft injury,discerning allograft rejection,and guiding immunosuppressant management.As a novel biomarker,dd-cfDNA is non-invasive,reproducible and highly sensitive.This article will review the recent research progress on the dd-cfDNA in kidney transplantation.