VRTN基因型对猪生长和繁殖性能的影响

Association of VRTN gene polymorphisms with growth and reproductive trait in pig

目的为了研究椎骨发育相关(vertnin,VRTN)基因对猪生长和繁殖性能的影响。方法利用PCR产物电泳法检测加系长白、加系大白、加系杜洛克及美系杜洛克母猪的VRTN基因多态性,以分析VRTN基因不同基因型对生长(校正100 kg背膘厚、校正100 kg体重日龄、校正100 kg眼肌面积等)和繁殖性能(繁殖指数、父系指数、母系指数、总仔数EBV、活仔数EBV等)的影响。结果在加系大白猪中,3种基因型间生长、繁殖性状无显著差异(P>0.05);在加系长白猪中,背膘厚qq基因型显著大于Qq基因型(P<0.05),qq基因型校正100 kg背膘厚EBV极显著高于Qq基因型(P<0.01)、显著高于QQ基因型(P<0.05);加系杜洛克猪中,QQ基因型校正100 kg体重日龄EBV显著大于qq基因型(P<0.05),母系指数qq基因型极显著高于Qq、QQ基因型(P<0.01),父系指数qq基因型极显著高于QQ基因型(P<0.01)、显著高于Qq基因型(P<0.05);在美系杜洛克猪中,qq基因型的校正100 kg体重日龄EBV显著大于Qq(P<0.05),母系指数和父系指数Qq基因型均极显著高于qq基因型(P<0.01)。结论VRTN基因对长白和杜洛克猪的生长和繁殖性能存在影响,为培育出多肋骨的商品猪提供了依据。

Objective The aim of this study is to investigate the polymorphism of vertnin(VRTN)and its association with the reproductive traits and the growth traits in sows.Method The polymorphism of VRTN genes of Canadian Yorkshire,Canadian Landrace,Canadian Duroc and American Duroc sows are detected by PCR method,and the association between different genotypes with the reproductive traits(100 kg of back fat,corrected for 100 kg of body weight and age,corrected for 100 kg,eye muscle area,etc)and the growth traits(reproduction index,paternal index,maternal index,total number of litters EBV,number of live litters EBV,etc)are investigated.Results There was no significant effects on the growth and the reproductive traits with different genotypes of VRTN in Canadian Yorkshire(P>0.05).The back-fat thickness of QQ genotype was higher than Qq genotype(P<0.05),the Corrected 100 kg backfat thickness EBV of qq genotype was significantly higher than that of Qq genotype(P<0.01)and higher than that of QQ genotype(P<0.05).The corrected 100 kg weight EBV of QQ genotype in Canadian Duroc sows was higher than that of qq genotype(P<0.05),the maternal index of qq genotype was significantly higher than that of Qq and QQ genotypes(P<0.01),for sire line index,qq genotype was significantly higher than that of QQ(P<0.01)and higher than that of QQ genotype(P<0.05).In American Duroc,the corrected EBV of 100 kg back-fat thickness in qq genotype was higher than that of Qq(P<0.05).Both maternal line index and sire line index Qq genotype were significantly higher than that of qq(P<0.01).Conclusions The polymorphism of VRTN gene affected the reproductive traits and growth traits in Canadian Landrace,Canadian Duroc and American Duroc sows,which provides a basis for breeding multi-rib commercial pigs.