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一种微酸性电解水的消毒相关性能研究

Disinfection performance of a kind of slightly acidic electrolyzed water
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摘要 目的 评价一种微酸性电解水的消毒性能,为实际应用提供依据。方法 选取现制微酸性电解水,用悬液定量杀菌试验、空气现场消毒试验方法、毒理试验和理化试验方法,在实验室观察该微酸性电解水的杀菌效果、毒性、腐蚀性和理化指标等。结果 该微酸性电解水的主要有效成分为次氯酸,平均有效氯含量为71 mg/L。用该微酸性电解水原液作用0.5 min,对悬液内金黄色葡萄球菌、大肠埃希菌和铜绿假单胞菌的杀灭对数值均>7.00,对悬液内白色念珠菌的杀灭对数值均>6.00。该微酸性电解水原液经气溶胶喷雾器雾化并作用30 min,对密闭空间的空气中自然菌平均消亡率>90%。用该微酸性电解水原液擦拭物体表面并保持作用5 min,对自然菌的杀灭对数值均>1.0。该微酸性电解水对小鼠急性经口毒性LD值>5 000 mg/(kg·bw),对小鼠急性吸入毒性试验LC2 h值>10 000 mg/m^(3);对小鼠骨髓嗜多染红细胞微核试验结果为阴性;对家兔的完整皮肤和眼刺激试验均属无刺激性。结论 该微酸性电解水原液对细菌繁殖体和真菌具有快速杀灭效果,属于无毒级物质,对皮肤黏膜无刺激作用。 Objective To understand the disinfection-related performance of a disinfectant produced by a slightly acidic electrolyzed water generator, and to provide a scientific basis for disinfection applications.Methods Suspension quantitative disinfection test, air field disinfection test, toxicological test and physical and chemical test were used to observe the bactericidal effect, toxicity, corrosiveness, physical and chemical indicators of the slightly acidic electrolyzed water made at present in the laboratory.Results The main effective component of the slightly acidic electrolyzed water was hypochlorous acid and the average available chlorine value was 71 mg/L.The logarithmic values were all> 5.00 for 0.5 min to kill Staphylococcus aureus,Escherichia coli,and Pseudomonas aeruginosa in the suspension, and the logarithmic values of Candida albicans in the liquid was> 4.00.The slightly acidic electrolyzed water stock solution was atomized by an aerosol sprayer and acted for 30 minutes, and the average extinction rate of natural bacteria in the air in a confined space was >90%.using this disinfectant to wash hands and scrub the skin surface for 1.0 min and 3.0 min respectively, and the logarithmic value of killing natural bacteria were both> 1.0;the surface of the object was wiped and disinfected for 5 minutes, and the logarithmic value of killing natural bacteria was >1.0. The LDvalue of the acute oral toxicity test for mice was >5 000 mg/kg(kg·bw);LC2 h value of the acute inhalation toxicity test for mice was >10 000 mg/m^(3), which was actually non-toxic.The result of the micronucleus test for mouse bone marrow polychromatic erythrocytes was negative.The skin irritation test and eye irritation test on rabbits were non-irritating.Conclusion The slightly acidic electrolyzed water stock solution has a rapid killing effect on bacterial propagules and fungi.It is non-toxicandnon-irritating for skin and mucosa.
作者 宋江南 尹进 高琼 夏昕 戴俊斌 刘美佟 陈贵秋 SONG Jiang-nan;YIN Jin;GAO Qiong;Xia Xin;DAI JUN-bin;LIU Mei-tong;CHEN Gui-qiu(Hunan Provincial Center for Disease Control and Prevention,Changsha Hunan 410005,China)
出处 《中国消毒学杂志》 CAS 2022年第10期737-739,742,共4页 Chinese Journal of Disinfection
关键词 微酸性电解水 有效氯 杀菌效果 毒性 腐蚀性 slightly acidic electrolyzed water available chlorine B bactericidal effect toxicity corrosive
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