食源性金黄色葡萄球菌的耐药分子分型研究

Antibiotic resistance molecular typing of foodborne Staphylococcus aureus

目的 研究食源性金黄色葡萄球菌的耐药分子分型特征。方法 采用微量肉汤稀释法,对食源性金黄色葡萄球菌的耐药性及其分子分型特征进行分析。结果 本研究共分析7株食源性金黄色葡萄球菌,对青霉素和氨苄西林的耐药率分别为100%和85.71%,对其他12种检测的抗菌药物均敏感。7株食源性金黄色葡萄球菌中有6株产肠毒素,4株产E型肠毒素,2株产C型肠毒素。PFGE带型分为4个型别,相似系数在38.5%~91.1%。结论 北京市食源的金黄色葡萄球菌耐药谱单一,肠毒素分布以产单一肠毒素为主。PFGE带型分布分散,与耐药谱和肠毒素分布的关联性不强。

Objective To study the antibiotic molecular typing characteristics of Staphylococcus aureus from ready to eat food.Methods The micro broth dilution was used to detect antibiotic resistance of S.aureus to antibiotics.Results 7 strains of S.aureus were 100% resistant to penicillin, 85.71% resistant to ampicillin and sensitive to other 12 antibiotics.6 strains of S.aureus produced enterotoxin, 4 strains produced type E enterotoxin and 2 strains produced type C enterotoxin.7 strains of S.aureus were divided into four types, and the similarity coefficient was 38.5%-91.1%.Conclusion The antibiotic resistance spectrum of S.aureus from ready to eat food in Beijing is relatively single, are resistant to penicillin antibiotics.The SE is mainly single enterotoxin.The PFGE bands are scattered, and there is no strong correlation with antibiotic resistance spectrum and SE.