池深和稀释比对肉种鸡精液品质的影响及稀释液筛选探究

Effects of Chamber Depth and Dilution Ratio on Semen Quality and Semen Diluent Screening for Broiler Breeder

为利用计算机辅助精液分析系统对种公鸡精液品质进行准确鉴定,筛选适合Ross 308肉种鸡的精液稀释液,试验通过分析不同制片池深(30μm、40μm、60μm、100μm)和稀释比(1∶40、1∶60、1∶80、1∶100)对精液品质的影响;在此基础上比较4种稀释液(Ⅰ、Ⅱ、Ⅲ、Ⅳ)对精子活力和运动参数的影响,确定稀释液的pH值和渗透压范围。结果显示:30μm和40μm的池深适合种鸡精液检测,60μm和100μm的池深会造成精子分层,精子不能成像于同一焦面,造成试验误差;稀释比显著影响精子活率和活力率(P<0.05),稀释比为1∶40和1∶60时,精子活率和活力率显著高于稀释比为1∶80和1∶100(P<0.05);稀释液种类对精子活率和活力率有显著影响(P<0.05),稀释液Ⅰ的精子活率和活力率分别为(91.33±5.13)%和(85.28±7.17)%,显著高于其他3种稀释液(P<0.05);稀释液pH为6.35~7.50、渗透压为320~350 mOsm/kg时可获得较好的精液稀释效果。综上所述,30μm和40μm制片池深及1∶40和1∶60的稀释比可作为检测种鸡精液品质的样品制作参数,与原精液渗透压相近的稀释液Ⅰ适用于Ross 308肉种鸡精液的稀释。

In order to get accurate semen quality identification using the computer-aided sperm analysis system and screen suitable semen diluent for Ross 308 broiler breeders, semen quality of test samples in different chamber depths(30 μm, 40 μm,60 μm, 100 μm) and dilution ratios(1∶40, 1∶60, 1∶80, 1∶100) were compared. Furthermore, the effects of four diluents(Ⅰ, Ⅱ,Ⅲ, Ⅳ) on sperm motility and movement parameters were studied base on selected sample preparation parameters, and got the pH and osmotic pressure competently. The results showed that sperm samples of 30 μm and 40 μm chamber depths were suitable for breeder sperm analysis, but 60 μm and 100 μm chamber depths had multiple layers and the images were not easy to focus, resulting in experimental error. Dilution ratios significantly affected sperm viability and motility(P<0.05). The viability rates and motility rates of the 1∶40 and 1∶60 dilution ratio groups were significantly higher than those of 1∶80 and 1∶100 groups(P<0.05). Diluents also had significant effects on viability and motility of diluted semen(P<0.05). The sperm viability rate and motility rate of diluent Ⅰ were(91.33±5.13) % and(85.28±7.17) % separately, which were significantly higher than those of the other three diluents(P<0.05). The diluents with the p H of 6.35 to 7.50 and the osmotic pressure of 320 mOsm/kg to 350 mOsm/kg were beneficial to obtain better semen dilution effect. In conclusion, semen test samples with the chamber depths of 30 μm or 40 μm and the dilution ratios of 1∶40 or 1∶60 were suitable for semen quality identification. Moreover, the osmotic pressure of diluent Ⅰ closed to that of the original semen of breeding birds was more suitable for Ross 308 parentstocks.