螺虫乙酯及其代谢物在石榴上的残留检测和膳食风险评估

Residual Detection and Dietary Risk Assessment of Spirotetramat and Its Metabolites in Punica granata L.

本文建立了螺虫乙酯及其4种代谢物在石榴上的残留检测方法,评估了螺虫乙酯在石榴中的残留量,进行了一般消费人群长期和短期膳食暴露评估。结果表明:螺虫乙酯及其代谢物的标准溶液在0.005~0.500 mg/L浓度范围内,其进样质量浓度与对应峰面积呈现出良好的线性关系,相关系数>0.99;当添加水平为0.01、0.10、1.00 mg/kg时,石榴中螺虫乙酯及其代谢物的平均回收率为74%~100%,相对标准偏差(RSD)为0.9%~5.3%,最低检测浓度为0.01 mg/kg;长期膳食暴露风险占每日允许摄入量(ADI)的比值(%ADI)为23.36%,短期膳食暴露风险占急性参考剂量(ARfD)的比值(%ARfD)为0.1464%。在本试验方案下,螺虫乙酯及其4种代谢物在石榴上的长期和短期膳食风险均<100%,对一般人群的身体健康不会产生不可接受的影响。

This paper established a method for residue determination of spirotetramat and its 4 kinds of metabolites in Punica granata L.,assessed residual spirotetramat in Punica granata L.and the long-term and short-term dietary expo-sure risk of the general consumer population.The results showed that when the standard solution concentration of spiro-tetramat and its 4 kinds of metabolites was in the range of 0.005-0.500 mg/L,there was a good linear relationship between the mass concentration and the corresponding peak area,the correlation coefficients(r2)were greater than 0.99.When concentrations were 0.01 mg/kg,0.10 mg/kg and 1.00mg/kg,the average recoveries of spirotetramat and its 4 kinds of metabolites in Punica granata L.ranged from 74%to 100%,and RSD ranged from 0.9%to 5.3%.The lowest concentra-tion was 0.01 mg/kg.The ratio of long-term dietary exposure risk to ADI(%ADI)was 23.36%,and the ratio of short-term dietary exposure risk to ARfD(%ARfD)was 0.1464%.According to the experimental protocol adopted in this study,the long-term and short-term dietary risk of spirotetramat and its 4 kinds of metabolites in Punica granata L.was less than 100%,which didn′t have unacceptable health effects on the general population.