摘要
【目的】对云南某动物园袋鼠粪样中分离到的1株隐孢子虫分离株进行种/基因型的分子鉴定,为云南动物园的袋鼠隐孢子虫病防控提供理论依据。【方法】针对隐孢子虫小亚基核糖体RNA基因序列设计引物,进行巢式PCR扩增,并对扩增产物进行测序和序列比对;使用DNAMAN和MEGA7.0等软件进行同源性分析并构建系统发育进化树,以确定该袋鼠源分离株与其他源隐孢子虫间的亲缘关系。【结果】成功扩增出隐孢子虫阳性片段。BLAST结果表明:获得的袋鼠源隐孢子虫分离株与鼠隐孢子虫(Cryptosporidium muris)一致,同源性可达100%。种系发育进化树表明:该分离株与鼠隐孢子虫处于同一分支,亲缘关系最近,属于胃寄生隐孢子虫的鼠隐孢子虫。【结论】该园袋鼠存在隐孢子虫感染,鉴定为具有人兽共患潜能的鼠隐孢子虫。本研究首次报道了中国袋鼠感染鼠隐孢子虫。
[Purpose]To identify the species/genotype of a Cryptosporidium isolated from faeces of kangaroos in a zoo in Yunnan,providing theoretical basis for the prevention and control of cryptosporidiosis in the zoo of Yunnan.[Methods]Primers were designed target to the conserved sequence of ribosomal RNA gene of Cryptosporidium for nested PCR amplification,and the PCR products was sequenced and compared.DNAMAN and MEGA 7.0 was used for homology analysis and phylogenetic tree was constructed to determine the genetic relationship between the kangaroo isolates and other Cryptosporidium.[Results]The positive fragment of Cryptosporidium was suc-cessfully amplified.BLAST results showed that the Cryptosporidium isolated from kangaroo was identical to Cryptosporidium muris,which was 100%homologous to C.muris.The phylogenetic tree showed that the isolated Cryptosporidium and C.muris were in the same genetic branch and most closely related,a gastric parasitic Cryptosporidium,and belonged to the gastro parasitic Cryptospor-idium category.[Conclusion]The kangaroo in this zoo shows the presence of Cryptosporidium in-fection and the pathogen is identified as C.muris,which has zoonosis potential.This is the first report of C.muris infection in kangaroo in China.
作者
衡昭君
马洲
董星和
程文杰
邹丰才
黄翠琴
贺君君
杨建发
HENG Zhaojun;MA Zhou;DONG Xinghe;CHENG Wenjie;ZOU Fengcai;HUANG Cuiqin;HE Junjun;YANG Jianfa(College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,China;Baoshan Animal Health Supervision Station,Baoshan 678000,China;College of Life Science,Longyan University,Fujian Provin-cial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology,Longyan 364000,China)
出处
《云南农业大学学报(自然科学版)》
CAS
CSCD
北大核心
2022年第6期999-1003,共5页
Journal of Yunnan Agricultural University:Natural Science
基金
云南省朱兴全专家工作站(202005AF150041)
云南农业大学兽医公共卫生省创新团队(202105AE160014)
福建省家畜疫病防治与生物技术重点实验室开放基金(2019KF03)。
关键词
袋鼠
隐孢子虫
分子鉴定
种系发育
云南
kangaroo
Cryptosporidium
molecular identification
phylogenesis
Yunnan