石豆兰杂交种无菌播种技术研究

Study on Aseptic Seeding of Bulbophyllum Hybrids Culture Technology

为建立石豆兰杂交种无菌播种快速繁殖体系,以‘Bulbophyllum pingtungense’为父本、‘Bulbophyllum fascinator var.corazonae’为母本进行种间杂交,取其F1代成熟种子作为试验材料,通过无菌播种技术对石豆兰杂交种子萌发、增殖、生根等问题进行研究。结果表明:最适于石豆兰杂交种子萌发的培养基为1/2MS+土豆汁100 g·L^(-1)+活性炭1 g·L^(-1);芽增殖最适宜的培养基为MS+6-BA1.0 mg·L^(-1)+NAA0.5 mg·L^(-1)+活性炭1 g·L^(-1),平均出芽数可达9.2个;壮苗生根的最佳培养基为1/2MS+NAA0.5 mg·L^(-1)+IBA1.0 mg·L^(-1)+活性炭1 g·L^(-1)+土豆汁100 g·L^(-1),生根率可达100%,平均根数为3.88条,平均株高达4.18 cm。综上,初步建立的石豆兰杂交种快速繁殖体系,可为石豆兰杂交种的种质资源保存提供理论和技术支持。

In this study,in order to establish a rapid propagation system for aseptic seeding of Bulbophyllum hybrids.'Bulbophyllum pingtungense'and'Bulbophyllum fascinator var.corazonae'were selected for interspecific hybridization.The mature seeds of F1 generation were used as experimental materials.The sterile sowing techniques were used to study germination,proliferation and rooting of Bulbophyllum hybrid seeds.The results showed that the optimum medium for aseptic germination of Bulbophyllum hybrid seeds was 1/2MS+potato juice 100 g·L^(-1)+activated carbon 1 g·L^(-1);the most suitable medium for the proliferation of buds was MS+6-BA1.0 mg·L^(-1)+NAA0.5 mg·L^(-1)+activated carbon 1 g·L^(-1),the proliferation coefficient of buds was 9.2.The optimal medium for rooting was 1/2MS+NAA0.5 mg·L^(-1)+IBA1.0 mg·L^(-1)+activated carbon 1 g·L^(-1)+potato juice 100 g·L^(-1),which the rooting rate was 100%.The average number of roots was 3.88,and the average plant height was 4.18 cm.In conclusion,this article established a rapid propagation technology system of Bulbophyllum hybrids,provided theoretical and technical support for germplasm resource conservation of Bulbophyllum hybrids.