一种免疫调节肽的小鼠经口急性毒性试验

Acute Oral Toxicity Test of an Immunomodulatory Peptide in Mice

试验采用急性毒性试验的限量法,将54只小鼠随机分为3组,Ⅰ、Ⅱ组为试验组,Ⅲ组为对照组。试验组Ⅰ、Ⅱ分别灌胃剂量200和400 mg/kg的免疫调节肽,对照组灌胃等量蒸馏水。结果表明,各试验组小鼠眼部、鼻孔、皮肤等器官均未出现临床中毒症状,经解剖观察发现各内脏器官生长发育正常,均无大体解剖病理变化,各试验组公鼠的生长性能以及小鼠血常规指标、血清生化指标和脏器系数与对照组相比均无显著差异(P>0.05);2个试验组母鼠末重、平均日增重显著高于对照组(P<0.05),各组小鼠始重、平均日采食量和料重比差异均不显著(P>0.05)。由此可见,该试验所用免疫调节肽对小鼠无急性毒性作用。

The limit method of the acute toxicity test was used in the experiment.54 mice were randomly divided into 3 groups,test groupⅠ,test groupⅡ,control group.The mice in test groupⅠand test groupⅡwere administrated with 200 and 400 mg/kg immunomodulatory peptide,the mice in the control group were administrated with the same dose of distilled water.The results showed that the eyes,nostrils,skin and other organs of the mice in each test group had no clinical symptoms of poisoning.The growth and development of various internal organs were observed by anatomy,and there were no general anatomical and pathological changes.The growth performance of the male mice and hematology routine indices,serum biochemical indices,and organ coefficients of mice in the test groups had no significant difference with those in the control group(P>0.05).The final body weight and average daily weight gain of the female mice in the two test groups were significantly higher than those in the control group(P<0.05).The initial body weight,average daily feed intake and feed-to-weight ratio had no significant difference between the groups(P>0.05).Therefore,the immunomodulatory peptide used in this experiment had no acute toxic effect on mice.