不同提取工艺的芪黄益气摄血方对免疫性血小板减少症模型小鼠治疗作用比较研究

Comparative study on therapeutic effects of Qihuang Yiqi Shexue Formula prepared by different extraction processes on ITP model mice

目的 比较筛选不同工艺提取制备的芪黄益气摄血方对免疫性血小板减少症(ITP)模型小鼠的治疗作用。方法 采用全方水提、水提醇沉、水提+醇提3种不同工艺提取芪黄益气摄血方,分别得工艺1组、2组、3组制备的试验样品;70只BALB/c小鼠随机分为7组,分别为正常组,模型组,芪黄益气摄血方工艺1组、2组、3组,维血宁颗粒组和醋酸泼尼松组,每组10只。采用豚鼠抗小鼠血小板血清(APS)腹腔注射方法,建立ITP小鼠模型;注射APS后的第8天,各组分别灌胃给药,连续灌胃14 d。观察比较各药物组对ITP模型小鼠一般情况、外周血血小板计数(PLT)、脾脏和胸腺指数、骨髓巨核细胞数的影响。结果 与正常组比较,模型组小鼠外周血PLT计数、Hb浓度显著下降(P <0.05或P <0.01),脾脏和胸腺指数显著升高(P <0.01),骨髓产板巨核细胞数明显减少(P <0.01);与模型组比较,3种工艺提取制备的芪黄益气摄血方均可明显提高ITP模型小鼠的PLT计数(P <0.01),以工艺1组、3组PLT升高明显,其中工艺3组接近正常组;在改善Hb水平方面,工艺1组、2组、3组均出现增加,但仅工艺3组有显著性差异(P <0.05);在降低脏器指数方面,工艺1组、3组脾脏和胸腺指数均出现显著降低(P <0.05或P <0.01),而工艺2组降低程度无统计学差异(P> 0.05);在改善骨髓巨核细胞数方面,工艺1组、2组、3组骨髓产板巨核细胞数均显著增加(P <0.01),以工艺3组增加最明显,其余指标未见统计学差异(P> 0.05)。阳性药维血宁颗粒和强的松组对上述指标均有不同程度的改善作用,而工艺3组在增加PLT计数、降低脾脏系数、增加骨髓巨核细胞数方面,均优于维血宁颗粒和强的松。结论 3种不同工艺提取制备的芪黄益气摄血方对ITP模型小鼠均有一定的治疗作用,治疗效果为工艺3组>1组> 2组。

Objective To compare and screen the therapeutic effects of Qihuang Yiqi Shexue Formula prepared by different processes on immune thrombocytopenia(ITP) model mice. Methods The Qihuang Yiqi Shexue Formula was extracted by three different processes: water extraction, water extraction and alcohol precipitation, water extraction alcohol extraction, and the test samples prepared by process 1, process 2, and process 3 were obtained respectively;70 BALB/c mice were randomly divided into 7 groups: normal group, model group, Qihuang Yiqi Shexue Formula process 1, process 2, process 3, Weixuening granules group and prednisone group, with 10 mice in each group. The intraperitoneal injection of guinea pig anti-mouse platelet serum(APS) was used to establish an ITP mouse model;on the 8th day after the injection of APS, each group was given intragastric administration for 14 consecutive days. To observe and compare the effects of each drug group on the general condition of ITP model mice, peripheral blood platelet count(PLT), spleen and thymus index, and bone marrow megakaryocyte count. Results Compared with the normal group, the PLT count and Hb concentration in the peripheral blood of the model group were significantly decreased(P<0.05 or P<0.01), the spleen and thymus index were significantly increased(P<0.01), and the number of megakaryocytes in the bone marrow plate was significantly reduced(P<0.01);compared with the model group, the Qihuang Yiqi Shexue Formula prepared by the three processes can significantly increase the PLT count of ITP model mice( P<0.01), and the PLT of the process group 1 and group 3 increased significantly, among which the process 3 groups are close to the normal group;in terms of improving the Hb level, there was an increase in the process 1, 2, and 3 groups, but only the process 3 group had a significant difference(P<0.05);in terms of reducing the organ index, the spleen and thymus indexes of the process 1 and 3 groups all decreased significantly(P<0.05 or P<0.01), but there was no statistical difference in the degree of reduction in the process 2 group(P>0.05);in terms of improving the number of bone marrow megakaryocytes, the number of bone marrow-producing megakaryocytes in the process 1, 2, and 3 groups all increased significantly(P<0.01), and the increase was the most obvious in the process 3 group, but there was no statistical difference in other indicators(P>0.05). The positive drugs Weixuening granules and the prednisone group have different degrees of improvement on the above indicators, while the process 3 group was better than Weixuening granules and prednisone in increasing the PLT count, reducing the spleen coefficient, and increasing the number of bone marrow megakaryocytes. Conclusion The Qihuang Yiqi Shexue Formula prepared by 3 different processes has a certain therapeutic effect on ITP model mice, and the therapeutic effect is process 3>process 1 >process 2.