双分子荧光互补技术在动物病毒中的研究进展

Progress of Bimolecular Fluorescence Complementation Technology in Animal Viruses Research

病毒侵染宿主的过程存在着一系列相互作用,了解病毒与宿主之间的蛋白质相互作用对于深入研究病毒具有重要意义。在众多研究蛋白质相互作用的方法中,双分子荧光互补技术(bimolecular fluorescence complementation,BiFC)因其能在活细胞中可视化相互作用而被广泛应用。介绍了双分子荧光技术的原理、发展和优势,总结了双分子荧光技术在动物病毒以及抗病毒药物研究中的应用,并进一步阐述了新型双分子荧光系统的原理,以期为研究动物病毒致病机制和抗病毒药物研发提供新的思路。

There are a series of protein-protein interactions in the process of virus infection.Therefore,understanding the interactions between the viruses and the hosts are of great significance for the in-depth studies of virology.Currently,there are many techniques used in protein interactions research,among which the bimolecular fluorescence complementation(BiFC)is widely used due to its ability to visualize interactions in living cells.This paper introduced the principle,development and advantages of BiFC technology.The application of BiFC technology in animal viruses and antiviral drugs were also summarized,and the principle of some new bimolecular fluorescence systems were further elaborated,so as to provide new ideas for the studies of virus pathogenic mechanism and the development of antiviral drugs.