痹通对佐剂性关节炎大鼠白介素17、转化生长因子β、叉头蛋白3表达的影响

Effect of Bi Tong on the expression of Interleukin-17(IL-17),Transforming growth factor-β(TGF-β),and Forkhead box protein(FOXP3)in adjuvant arthritis rats

目的探讨中药痹通(BT)对佐剂性关节炎(AA)大鼠白介素17(IL-17)、转化生长因子β(TGF-β)、叉头蛋白P3(Foxp3)及沉默调节蛋白1(SIRT1)表达影响,并分析其治疗作用机理。方法建立AA大鼠模型,分别以痹通高、中、低剂量为干预因素,观测大鼠踝关节病理,酶联免疫吸附法(Elisa)检测外周血IL-17、TGF-β表达,免疫组织化学法(IHC)检测踝关节Foxp3表达、免疫荧光法(IF)检测踝关节SIRT1表达。结果BT高、中、低剂量组IL-17的表达显著降低(P<0.01),TGF-β、Foxp3、SIRT1的表达显著增加(P<0.05);痹通高、中、剂量对AA大鼠踝关节病理评分结果有显著改善(P<0.05)。结论痹通对AA大鼠足踝关节肿胀有显著改善作用,其机理可能与调节辅助性T细胞17/调节性T细胞(Th17/Treg)平衡有关。

Objective To investigate the effect of Bi Tong(BT)on the expression of IL-17, TGF-β, Foxp3 and SIRT1 in adjuvant arthritis(AA) rats, and to analyze its therapeutic mechanism.Methods AA Rat model was established, and the pathological changes of ankle joint were observed with high, middle and low dose of Bi Tong. IL-17 and TGF-β expression in peripheral blood were detected by Enzyme linked immunosorbent assay,( ELISA) way, Foxp3 was detected by Immunohistochemistry(IHC) method, and SIRT1 was observed by Immunofluorescence(IF) method. Results The expression of IL-17 was significantly decreased(P<0.01), and the expression of TGF-β, Foxp3 and SIRT1 were significantly increased(P<0.05) in high, middle and low doses of Bi Tong(P<0.01). The ankle pathological index were significantly improved(P<0.05) in three BT groups.Conclusion BT can significantly improve the swelling of ankle joint in AA Rats, and the mechanism might be related to the regulation of Th17/Treg balance.