输尿管支架表面化学接枝镀铜涂层及其性能

Preparation and Properties of Copper-carrying Polydopamine Coating on Ureteral Stent

采用聚多巴胺接枝化学镀铜方法在316L不锈钢表面制备载铜聚多巴胺涂层,使用扫描电镜、原子力显微镜、X射线光电子能谱、电感耦合等离子体质谱仪等手段表征其表面形貌、成分和铜离子释放量并将其与细菌和细胞共培养,研究了涂层的抗感染、抗结石性能和生物相容性。结果表明,载铜涂层的厚度为27 nm,分布均匀,其中的铜以Cu、CuO和CuO的形式存在。将涂层在人工尿液中浸泡14 d,铜离子每天的释放量接近。将涂层分别与大肠杆菌和金黄色葡萄球菌培养24 h后,抗菌率为96.2%和95.9%。在金黄色葡萄球菌悬液中浸泡30 d后在涂层表面沉积的钙离子和镁离子含量分别为48.7 mg/L和235.3 mg/L,明显低于对照组。细胞增殖实验的结果表明,这种涂层无细胞毒性。

Bacteria adhesion and encrustation formation on the surface of ureteral stents have been common complications clinically. Herewith, the Cu grafting polydopamine coating was prepared on 316L stainless steel via polydopamine graft agent assisted electroless Cu plating method, in order to create a coating with performance of anti-infection, anti-stone formation and good biocompatibility for the ureteral stent surface. While the surface morphology, composition and copper ions release of the coatings were assessed by means of scanning electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy and inductively coupled plasma mass spectrometry. Samples were incubated with bacteria and cells respectively, so that to examine the antibacterial ability, encrustation resistance and biocompatibility of coatings. The results show that the coating is evenly distributed. The copper in the coating consists of Cu, CuO and CuO and its thickness is 27.0 nm. The content of releasing copper for each day remains nearly constant after immersion in artificial urine. The antibacterial rates against Escherichia coli and Staphylococcus aureus are 96.2% and 95.9% after incubating for 24 hours, respectively. The contents of calcium and magnesium of samples, which are deposited in the human urine coupled with Staphylococcus aureus for 30 days, are 48.7 mg/L and 235.3 mg/L, respectively, which is significantly lower than that of the control group. Cells proliferation assay shows no cytotoxicity.