不同厂家酶联免疫试剂盒检测饲料中霉菌毒素效果评价

Evaluation of Enzyme-linked Immunosorbent Assay Kits from Different Manufacturers for Mycotoxin Detection in Feed

为了对市售6个厂家的酶联免疫霉菌毒素试剂盒进行综合评价,笔者选择6个厂家的黄曲霉毒素B1、玉米赤霉烯酮、呕吐毒素3种霉菌毒素试剂盒,检测饲料产品及饲料原料,分别以标准曲线的拟合程度、批次间的稳定性及与高效液相色谱法检测结果比对3个指标来评价试剂盒。E厂家的玉米赤霉烯酮试剂盒线性系数r^(2)≤0.99,其余厂家均≥0.99;6个厂家中,没有1个厂家3种试剂盒在不同样品中的批次间RSD均<10%;与高效液相色谱法检测结果比较,表明所有试剂盒回收率参差不齐。以上结果说明,实验室在选择试剂盒前一定要进行评估实验,并选择适合样品的试剂盒产品。

The mycotoxin enzyme-linked immunoassay kits from 6 manufacturers on the market were evaluated comprehensively.Methods Three mycotoxins(including Deoxynivalenol,Aflatoxin B1 and Zearalenone)kits from 6 manufacturers were selected to test two kinds of feed materials and feeds,and the three indexes of standard curve fitting degree,stability between batches and the difference of detection results between the ELISA kits and high performance liquid chromatography were evaluated respectively.The standard curve r^(2)1value of Zearalenone kit from manufacturer E did not reach 0.99,and the other manufacturers were all greater than 0.99.None of the six manufacturers had RSD<10%between batches in the three mycotoxin kits.The results of ELISA kits are uneven compared with high performance liquid chromatography.The laboratory must do the evaluation experiment before selecting the kit,and is suitable for the sample.