人结直肠癌组织及LOVO细胞中ARTC1的表达及其与肿瘤微血管生成的相关性

Expression of ARTC1 in human colorectal cancer tissues and LOVO cells and its relationship with microangiogenesis

目的探讨人结直肠癌组织及LOVO细胞中ARTC1对VEGF及bFGF的表达及微血管生成的影响。方法采用免疫组化、免疫荧光双标法检测结直肠癌组织中ARTC1、VEGF和bFGF的表达,并行微血管密度(microvessel density,MVD)分析。采用流式细胞术、Western blot法检测MIBG处理前后人结直肠癌LOVO细胞中ARTC1、VEGF和bFGF的表达差异。结果结直肠癌组织中ARTC1、VEGF和bFGF的阳性率分别为71.3%、85%、65%,明显高于正常对照组,且VEGF、bFGF的表达与ARTC1表达呈正相关(P<0.05)。ARTC1+VEGF(25.4±8.3)及ARTC1+bFGF(22.3±5.9)共表达组的MVD与非共表达组相比明显增加,差异有显著性(P<0.01)。LOVO细胞经MIBG处理前后,VEGF及bFGF阳性率分别由99.5%、91.38%降至77.84%、33.45%,差异有统计学意义(P<0.01);MIBG处理前VEGF(1.19±0.025)、bFGF(1.18±0.048)蛋白表达水平与处理后VEGF(0.71±0.053)、bFGF(0.58±0.033)相比明显减弱,差异有显著性(P<0.01)。结论ARTC1可能通过调节VEGF和bFGF的合成及其表达参与结直肠癌血管的生成。

Purpose To investigate the effect of ARTC1 on the expression of VEGF and bFGF and its relationship with microangiogenesis in colorectal cancer(CRC)tissues and LOVO cells.Methods Immunohistochemistry and immunofluorescence were used to detect the expression of ARTC1,VEGF and bFGF in CRC,and the microvessel density(MVD)analysis was performed.Flow cytometry and Western blot were used to detect the differences in expression of ARTC1,VEGF and bFGF in LOVO cells before and after MIBG treatment.Results The positive expression rates of ARTC1,VEGF and bFGF in CRC were 71.3%,85%and 65%respectively,which were significantly higher than those in normal controls and the expression of VEGF and bFGF was positively correlated with that of ARTC1(P<0.05).MVD was significantly increased in the co-expression group of ARTC1/VEGF(25.4±8.3)and ARTC1/bFGF(22.3±5.9)compared to the non-co-expression group(P<0.01).The percentage of VEGF and bFGF positive cells in LOVO cells before and after MIBG treatment was significantly reduced from 99.5%and 91.38%to 77.84%and 33.45%,respectively(P<0.01).The protein expression levels of VEGF(1.19±0.025)and bFGF(1.18±0.048)before MIBG treatment were significantly reduced compared with those of VEGF(0.71±0.053)and bFGF(0.58±0.033)after treatment,and the difference was significant(P<0.01).Conclusion ARTC1 may participate in the angiogenesis of CRC by regulating the synthesis and expression of VEGF and bFGF.