UCNP@mSiO_(2)纳米载体及其近红外光触发的药物可控释放研究

UCNP@mSiO_(2) nanocarriers and their controlled drug release triggered by near infrared light

为解决纳米载药体系在药物传递过程中不可控释放的问题,设计了一种近红外光(NIR)触发的光控药物释放体系。首先通过合成上转换纳米粒子(UCNPs),并在其表面包覆介孔二氧化硅(mSiO_(2)),得到UCNP@mSiO_(2);然后将偶氮苯(AZO)分子接枝到UCNP@mSiO_(2)表面,并在mSiO_(2)的介孔上负载阿霉素(DOX);最后加入环糊精(β-CD),得到纳米载药体系DOX-UCNP@mSiO_(2)@AZO@β-CD。对纳米载药体系的形貌和结构进行表征,并研究其在NIR照射下的药物可控释放行为。结果表明:药物释放速率随NIR光照强度的增加而增加,而在无NIR照射时药物基本不释放;细胞毒性实验证实纳米载药体系在无NIR照射时具有良好的生物相容性,而在NIR照射后显示出高的细胞毒性,表明负载的DOX在NIR照射下被释放并实现了杀死癌细胞的目的。该纳米载药体系具有优良的NIR触发的光控药物释放行为,在药物递送领域具有一定的应用价值。

To solve the problem of uncontrolled drug release of nanocarrier system in drug delivery process,a controlled drug release system triggered by near infrared light(NIR)was designed in this work.First of all,UCNP@mSiO_(2) was obtained by synthesizing the up-conversion nanoparticles UCNPs and coating them with mesoporous silica(mSiO_(2)).After that,the azobenzene(AZO)molecules were grafted to the surface of UCNP@mSiO_(2) and then doxorubicin(DOX)was loaded into the mesopores of mSiO_(2).Finally,β-cyclodextrin(β-CD)was added to obtain the nanocarrier system DOX-UCNP@mSiO_(2)@AZO@β-CD.The morphology and structure of the nanocarrier system were characterized and its controlled drug release behavior under NIR irradiation was examined in detail.The results showed that the drug release rate was accelerated with the increase of NIR irradiation intensity.In addition,basically no drug was released in the absence of NIR irradiation.The cytotoxicity test verified that the nanocarrier system showed good biocompatibility without NIR irradiation,but exhibited high cytotoxicity with NIR irradiation,indicating that the loaded DOX was released under NIR irradiation and killed cancer cells.The above results suggested that this nanocarrier system has excellent photocontrolled drug release triggered by NIR and a potential application value in the field of drug delivery.