扶正消瘿汤对实验性自身免疫性甲状腺炎大鼠的干预及免疫调节作用

Intervention and immune regulation of Fuzheng Xiaoying Decoction on experimental autoimmune thyroiditis model rats

目的:讨论扶正消瘿汤对于实验性自身免疫性甲状腺炎(experimental autoimmune thyroiditis,EAT)模型大鼠的治疗效果及调节免疫的作用机理。方法:选取雌性SD大鼠40只,随机数字法选取10只做空白组,其余大鼠使用弗氏佐剂与猪甲状腺球蛋白免疫注射同时结合高碘饮水造EAT模型,成模后随机平均分3组:模型组、硒酵母片组、扶正消瘿汤组,连续灌胃2个月。测定大鼠抗甲状腺抗体、甲状腺功能、IL-38及脾脏Th17、Treg细胞,观察甲状腺组织病理。结果:模型组抗甲状腺抗体TPOAb、TGAb水平高于空白组(P<0.05),淋巴细胞浸润甲状腺组织,滤泡内胶质分布不均匀且部分被破坏,提示造模成功。硒酵母片组与扶正消瘿汤组干预后TPOAb和TGAb水平明显低于模型组(P<0.05),扶正消瘿汤改善甲状腺滤泡破坏及淋巴细胞浸润的作用较硒酵母片组更明显(P<0.05)。与空白组对比,模型组大鼠T3、FT3、T4、FT4水平升高,IL-38水平降低,Th17%升高,Th17/Treg升高(P<0.05)。与模型组对比,硒酵母片组与扶正消瘿汤组的T4水平降低、IL-38水平升高(P<0.05),但Th17%以及Th17/Treg的降低差异无统计学意义(P>0.05)。各组间Treg%比较差异无统计学意义(P>0.05)。结论:扶正消瘿汤能通过调节EAT大鼠免疫,明显降低抗甲状腺抗体水平,改善甲状腺组织淋巴细胞浸润和滤泡破坏。

Objective:To investigate the effect of Fuzheng Xiaoying Decoction on experimental autoimmune thyroiditis(EAT)model rats and explore its possible mechanism of immune regulation.Methods:Among the 40 female SD rats,10 were randomly selected as the blank group,and the rest were immunized with pig thyroglobulin and Freund's adjuvant combined with high iodine feeding to make the EAT model.After the model was established,they were randomly divided into three groups:the model group,the selenium yeast tablet group and the Fuzheng Xiaoying Decoction group,which were continuously gavaged for 2 months.The anti thyroid autoantibodies,thyroid function,IL-38 and Th17/Treg distribution were measured.The pathological changes of thyroid tissue were observed by HE staining.Results:Compared with the blank group,the levels of TPOAb and TGAb in the model group were significantly higher(P<0.05).Lymphocyte infiltration was seen in the thyroid tissue of the model group,and also the thyroid follicles were partially destroyed,the shape was irregular,and the colloid distribution was uneven,indicating that the modeling was successful.Compared with the model group,the levels of TPOAb and TGAb in the selenium yeast tablet group and the Fuzheng Xiaoying Decoction group decreased significantly(P<0.05).And the Fuzheng Xiaoying Decoction group had more significant improvement in thyroid follicle destruction and lymphocyte infiltration than the selenium yeast tablet group(P<0.05).Compared with the blank group,the levels of T3,T4,FT3 and FT4 in the model group increased,the level of IL-38 decreased,the proportion of Th17 increased,and the ratio of Th17/Treg increased(P<0.05).Compared with the model group,T4 level decreased,IL-38 level increased in the selenium yeast tablet group and the Fuzheng Xiaoying Decoction group(P<0.05).But there was no statistically significant difference in the reduction of Th17 proportion and Th17/Treg ratio between the above two groups and the model group(P>0.05).There was no significant difference in Treg ratio among the groups.Conclu⁃sion:Fuzheng Xiaoying Decoction can significantly reduce the level of anti thyroid autoantibodies in EAT rats by regulating the im‐munity of EAT rats,and improve the follicular destruction and lymphocyte infiltration of thyroid tissue in rats.