基于“肾⁃血⁃骨”理论探究GH/IGF⁃1系统在青娥丸防治骨质疏松症中的作用机制

Based on the theory of“Kidney-blood-bone”to explore the mechanism of GH/IGF-1 system in the prevention and treatment of osteoporosis in Qing'e pill

目的:通过观察大鼠血、肾及骨组织生长激素(GH)/胰岛素样生长因子-1(IGF-1)系统的变化,探讨青娥丸对肾虚骨质疏松症的防治作用及机制,为研究肾虚骨质疏松症的病因病机提供依据。方法:大鼠骨质疏松症模型采用去卵巢法建立,将其随机依次分为正常组、模型组、西药组、健脾组、青娥丸高剂量组、青娥丸中剂量组、青娥丸低剂量组,灌胃给药8周后取材,ELISA法检测血清碱性磷酸酶(ALP),抗酒石酸酸性磷酸酶(TRACP),以及肾、骨和血中生长激素(GH)、生长激素受体(GHR)、胰岛素样生长因子-1(IGF-1)及胰岛素样生长因子-1受体(IGF-1R)的含量,所得数据用SPSS 25.0软件进行统计分析。结果:与正常组相比,模型组大鼠血清TRACP、ALP含量显著增加,提示大鼠发生高转换性骨质疏松;与正常组相比,模型组大鼠血、骨及肾组织GHR、IGF-1及IGF-1R的含量显著下降;与模型组相比,青娥丸高、中、低剂量组血清TRACP、ALP含量显著降低;与模型组相比,青娥丸高、中、低剂量组血、骨及肾组织GHR、IGF-1及IGF-1R的含量升高。结论:青娥丸能够改善骨质疏松症骨代谢情况;青娥丸可能是通过提高血、骨及肾中GHR、IGF-1及IGF-1R的含量实现对骨质疏松症防治。

Objective:To observe the changes of growth hormone(GH)/insulin-like growth factor-1(Igf-1)system in blood,kidney and bone tissue of rats,and to explore the preventive and therapeutic effect of Qing'e pill on osteoporosis due to kidney deficiency and its mechanism,to provide the basis for studying the etiology and pathogenesis of osteoporosis due to kidney deficiency.Methods:The rat osteoporosis model was established by ovariectomy,they were randomly divided into normal group,model group,Western Medicine Group,Jianpi Group,high-dose group,middle-dose group and low-dose group,the levels of serum alkaline phosphatase(ALP),tartrate acid phosphatase(TRACP),growth hormone(GH)and GHR(Ghr)in kidney,bone and blood were determined by Elisa,the contents of insulin-like growth factor-1(Igf-1)and insulin-like growth factor-1 receptor(IGF-1R)were analyzed by SPSS 25.0 software.Results:1.Compared with the normal group,the contents of TRACP and ALP in serum of the model group were significantly increased,suggesting that the rats developed high-conversion osteoporosis.2.The levels of serum,bone and renal GHR,Igf-1 and insulin-like growth factor-1 receptor(IGF-1R)in the model group were sig‐nificantly lower than those in the control group.3.Compared with the model group,the contents of TRACP and ALP in the high,middle and low dose groups were significantly decreased.4.Compared with the model group,the contents of GHR,Igf-1 and IGF-1R in blood,bone and kidney tissue of the high,middle and low dose groups were increased.Conclusion:1.Qing'e pill can improve the bone metabolism of osteoporosis.2.Qinge pill may prevent and cure osteoporosis by increasing the contents of GHR,Igf-1 and IGF-1R in blood,bone and kidney.