耐盐深绿木霉TW320和拟康宁木霉TW1876生长和产孢条件优化

Optimization of growth and spore-producing conditions of salt-tolerant Trichoderma atroviride TW320 and Trichoderma koningiopsis TW1876

为优化两株耐盐木霉-深绿木霉(Trichoderma atroviride)TW320和拟康宁木霉(Trichoderma koningiopsis)TW1876的生长和产孢条件,研究了不同pH、不同培养基,不同碳源、氮源和不同碳氮比对两株木霉菌的菌落生长和孢子产量的影响。结果显示:耐盐深绿木霉(T.atroviride)TW320在pH=4~11的马铃薯葡萄糖琼脂(potato dextrose agar, PDA)培养基上均可生长和产孢,且菌丝生长和产孢的最佳pH为5,在大豆玉米粉培养基上生长速度较快,产孢量最多,该菌株菌丝生长和产孢的最佳碳源为木糖,最佳氮源是酵母膏,当碳氮比为12:1时,最有利于TW320产孢;拟康宁木霉(T.koningiopsis)TW1876菌丝生长和产孢的最佳pH为11,最优培养基为PDA培养基,以乳糖作为最佳碳源,甘氨酸作为最佳氮源,且碳氮比为9:1时,TW1876产孢量最多。

The effects of different pH, medium, carbon source, nitrogen source, and C/N ratios on the colony growth and spore yield of two salt-tolerant Trichoderma strains, Trichoderma atroviride TW320 and Trichoderma. koningiopsis TW1876, were studied in order to optimize the growth and spore production conditions of the two strains. The results showed that the salt-tolerant T. atroviride TW320 could grow and produce spores on potato dextrose agar(PDA) medium with a pH of 4 to 11, and the optimum pH for mycelial growth and spore production was 5. On soybean corn flour medium, TW320 grew the fastest and produced the most spores. The best carbon source for TW320 mycelial growth and sporulation was xyaccharide, respectively, and the best nitrogen source for TW320 was yeast extract. When the C/N ratio is 12:1, TW320 produces the most spores. The optimum pH for mycelial growth and sporulation of T. koningiopsis TW1876 was 11, and the optimal medium was PDA. TW1876 has the highest sporulation capacity, with lactose being the best carbon source, glycine being the best nitrogen source, and the C/N ratio being 9:1.