电纺漆酶催化降解水中双酚A性能研究

Catalytic degradation performance of electrospun laccase for bisphenol A in water

以模拟地表水中的双酚A(BPA)为目标污染物,采用聚乳酸-乙醇酸共聚物(PDLGA)作为纺丝材料,利用静电纺丝固定化漆酶制作载漆酶电纺纳米纤维膜,考察了BPA溶液降解效果,分析了温度、pH等影响因素。结果表明:电纺纳米纤维膜固定化漆酶对游离酶的活性保留率为78.5%;固定化漆酶对低浓度BPA(100μg/L)和高浓度BPA(100 mg/L)的2 h降解率分别达到83.5%和31.7%;在温度为5~45℃的变化范围内,固定化酶和游离酶对BPA的降解率变化区间分别为31.2%~71.2%和29.8%~89.4%;在pH为1~9的变化范围内,固定化酶和游离酶对BPA的降解率变化区间分别为12.2%~64.7%和0~59.8%。

Taking bisphenol A(BHA)in simulated surface water as the target pollutant,using PDLGA as spinning materials,electrospun laccase-carrying nanofiber membranes were prepared with electrospinning immobilized laccase.The degradation performance of BPA was investigated.The influencing factors such as temperature and pH were analyzed.The results showed that the activity retention rate of laccase immobilized on electrospun nanofiber membrane to free enzyme was 78.5%.The two-hour degradation efficiencies of low concentration(100μg/L)and high concentration(100 mg/L)of BPA by immobilized laccase reached 83.5%and 31.7%,respectively.Within the temperature range of 5~45℃,the ranges of degradation efficiency of BPA by immobilized enzyme and free enzyme were 31.2%~71.2%and 29.8%~89.4%,respectively.Within the pH range of 1~9,the ranges of degradation efficiency of BPA by immobilized enzyme and free enzyme were 12.2%~64.7%and 0~59.8%,respectively.