血清抗微生物抗体检测对IBD的鉴别诊断价值分析

Analysis of the value of serum anti-microbial antibody detection in the differential diagnosis of IBD

目的分析血清抗微生物抗体检测对炎症性肠病(IBD)的鉴别诊断价值。方法以2018年5月至2020年5月收治的100例IBD患者为试验组,其中50例克罗恩病(CD)患者为CD组,50例溃疡性结肠炎(UC)患者为UC组,同时选取50例健康体检者为对照组。三组均给予血清抗微生物抗体检测。比较三组的血清抗细菌鞭毛蛋白抗体(Anti-CBirl)、抗细菌外膜孔道蛋白C抗体(Anti-Omp C)、抗荧光假单胞菌相关序列I2抗体(Anti-I2)、抗酿酒酵母抗体(ASCA)的阳性率,并分析Anti-CBirl、Anti-Omp C、Anti-I2、ASCA单独及四者联合对CD、UC的鉴别诊断价值。结果试验组的Anti-CBirl、Anti-I2、ASCA阳性率均高于对照组,CD组的Anti-Omp C阳性率高于UC组(P<0.05)。Anti-CBirl、Anti-Omp C、Anti-I2、ASCA联合诊断CD的灵敏度、特异度均高于Anti-CBirl、Anti-Omp C、Anti-I2、ASCA单独诊断(P<0.05)。Anti-CBirl、Anti-Omp C、Anti-I2、ASCA联合诊断UC的灵敏度、特异度均高于Anti-CBirl、Anti-Omp C、Anti-I2、ASCA单独诊断(P<0.05)。结论血清抗微生物抗体联合检测能够更好地鉴别诊断IBD患者的疾病类型。

Objective To analyze the value of serum anti-microbial antibody detection in the differential diagnosis of inflammatory bowel disease(IBD).Methods From May 2018 to May 2020,100 patients with IBD were selected as experimental group,including 50 patients with Crohn's disease(CD)as CD group and 50 patients with ulcerative colitis(UC)as UC group.At the same time,50 healthy objects were selected as control group.Serum anti-microbial antibodies were detected in all three groups.The positive rates of serum anti-bacterial flagellin(Anti-CBirl),Anti-outer membrane porin C(Anti-Omp C),anti-Pseudomonas fluorescens associated sequence I2(Anti-I2)and anti-saccharomces cerevisiae antibody(ASCA)in the three groups were compared,and the differential diagnosis values of Anti-CBirl,Anti-Omp C,Anti-I2,ASCA single and their combination for CD and UC were analyzed.Results The positive rates of Anti-CBirl,Anti-I2 and ASCA in the experimental group were higher than those in the control group,and the positive rate of Anti-Omp C in the CD group was higher than that in the UC group(P<0.05).The sensitivity and specificity of combined Anti-CBirl,Anti-Omp C,Anti-I2 and ASCA in diagnosis of CD were higher than those of Anti-CBirl,Anti-Omp C,Anti-I2 and ASCA single diagnosis(P<0.05).The sensitivity and specificity of combined Anti-CBirl,Anti-Omp C,Anti-I2 and ASCA in diagnosis of UC were higher than those of Anti-CBirl,Anti-Omp C,Anti-I2 and ASCA single diagnosis(P<0.05).Conclusion Combined detection of serum anti-microbial antibodies can better differentiate the disease types of IBD patients.