补肾活血法改善雷公藤甲素致大鼠卵巢毒性的效果研究

Effect of tonifying kidney and promoting blood circulation on ovarian toxicity induced by triptolide in rats

目的:以雷公藤甲素致大鼠卵巢毒性模型为载体,探索补肾活血法对其大鼠卵巢毒性的干预效果。方法:将有正常动情周期的SPF级SD雌性大鼠随机分为4组:正常组、雷公藤甲素模型组(雷公藤甲素50μg·kg^(-1)/d)、中药低剂量治疗组(雷公藤甲素50μg·kg^(-1)/d+淫羊藿苷30 mg·kg^(-1)/d+三七总皂苷30 mg·kg^(-1)/d)、中药高剂量治疗组(雷公藤甲素50μg·kg^(-1)/d+淫羊藿苷60 mg·kg^(-1)/d+三七总皂苷60 mg·kg^(-1)/d),每组6只,共24只,连续给药6个月。巴氏染色阴道脱落细胞涂片了解动情周期,称重计算卵巢子宫指数,留取卵巢行常规病理染色并计数各级卵泡。结果:正常组大鼠动情周期为4~5 d,模型组均出现动情周期紊乱,中药低剂量治疗组和中药高剂量治疗组均有半数以上大鼠动情周期正常。卵巢指数方面,模型组的卵巢指数低于正常组,差异有统计学意义(P<0.05);中药低剂量治疗组和中药高剂量治疗组的卵巢指数高于模型组,但差异无统计学意义(P>0.05)。子宫指数方面,四组比较,差异无统计学意义(P>0.05)。模型组原始卵泡、黄体数量低于正常组,闭锁卵泡高于正常组,但差异无统计学意义(P>0.05);模型组生长卵泡低于正常组,差异有统计学意义(P<0.05)。中药低剂量治疗组原始卵泡、黄体数量高于模型组,但差异无统计学意义(P>0.05);中药低剂量治疗组生长卵泡高于模型组,闭锁卵泡低于模型组,差异有统计学意义(P<0.05)。中药高剂量治疗组原始卵泡、生长卵泡数量高于模型组,但差异无统计学意义(P>0.05);中药低和高剂量治疗组闭锁卵泡低于模型组,差异有统计学意义(P<0.05)。结论:补肾活血法可有效降低雷公藤甲素对大鼠的卵巢毒性。

Objective The ovarian toxicity model of SD rats induced by triptolide was used as the carrier to explore the intervention effect of tonifying kidney and promoting blood circulation on ovarian toxicity.Methods SPF female SD rats with normal oestrous cycle were randomly divided into 4 groups:Normal group,triptolide model group[triptolide 50(μg·kg^(-1))/d],TCM low-dose treatment group[triptolide 50(μg·kg^(-1))/d+icariin 30(mg·kg^(-1))/d+total saponins of Panax notoginseng 30(mg·kg^(-1))/d),TCM highdose treatment group(triptolide 50(μg·kg^(-1))/d+icariin 60(mg·kg^(-1))/d+total saponin of Panax notoginseng 60(mg·kg^(-1))/d],6 rats in each group,a total of 24 rats,were given continuous administration for 6 months.Pap staining vaginal cells smear to understand the estrus cycle,weighing and calculating ovarian and uterine index,routine pathological staining of the ovaries and counting of follicles at all levels.Results The estrous cycle of rats in normal group is 4~5 days,and the estrous cycle disorder occurs in model group.More than half of rats in low-dose and high-dose TCM treatment groups have normal estrous cycle.Compared with the normal group,the ovarian index of the model group decreased,the number of primitive follicles,growing follicles and luteal body decreased,while the number of atretic follicles increased.Compared with the model group,the ovarian index of the low dose and high dose TCM treatment groups increased,and the number of primitive follicles as well as growing follicles increased,while the number of atretic follicles decreased.Conclusions Early use of Chinese herbal medicine for tonifying kidney and promoting blood circulation could effectively reduce the ovarian toxicity of triptolide.