阿魏酸钠对肺结核模型小鼠免疫功能及肺泡巨噬细胞吞噬功能的调控作用研究

Study on the Regulatory Effect of Sodium Ferulate on the Immune Function and Phagocytic Function of Alveolar Macrophages in Pulmonary Tuberculosis Model Mice

目的:探讨与分析阿魏酸钠对肺结核模型小鼠免疫功能及肺泡巨噬细胞吞噬功能的调控作用。方法:肺结核模型小鼠(n=36)随机分为模型组、利福平组、阿魏酸钠组,每组各12只。利福平组、阿魏酸钠组分别给予100 mg/kg剂量的利福平与阿魏酸钠,模型组小鼠灌胃等量生理盐水,1次/d,给药6周,观察与记录所有小鼠的一般特征;分别于给药第2周、第4周、第6周,HE染色观察小鼠的病理特征;MDA检测试剂盒和总SOD活性检测试剂盒测定肺组织MDA水平与SOD活性;流式细胞仪检测小鼠T淋巴细胞亚群-CD3^(+)T淋巴细胞、CD4^(+)T淋巴细胞水平;酶联免疫法检测血清IL-6、IL-8含量;AnnexinV-FITC检测肺泡巨噬细胞凋亡率。结果:利福平组、阿魏酸钠组给药第2周、第4周、第6周的肺组织丙二醛(Malondialdehyde,MDA)水平低于模型组(P<0.05),超氧化物岐化酶(superoxide dismutase,SOD)活性高于模型组(P<0.05),利福平组与阿魏酸钠组对比也有明显差异(P<0.05)。利福平组、阿魏酸钠组给药第2周、第4周、第6周的血液CD3^(+)T淋巴细胞、CD4^(+)T淋巴细胞比例明显高于模型组(P<0.05),阿魏酸钠组也高于利福平组(P<0.05)。利福平组、阿魏酸钠组给药第2周、第4周、第6周的血清IL-6、IL-8含量明显低于模型组(P<0.05),阿魏酸钠组也低于利福平组(P<0.05)。利福平组、阿魏酸钠组给药第2周、第4周、第6周的肺泡灌洗液(broncho alveolar lavage fluid,BALF)巨噬细胞凋亡率明显低于模型组(P<0.05),阿魏酸钠组也明显低于利福平组(P<0.05)。结论:阿魏酸钠在肺结核模型小鼠的应用可抑制炎症因子的表达,并改善氧化状况,还能增强小鼠的免疫功能,降低肺泡灌洗液巨噬细胞凋亡率。

Objective:To explore and analysis the regulatory effect of sodium ferulate on the immune function and phagocytic function of alveolar macrophages in pulmonary tuberculosis model mice.Methods:Pulmonary tuberculosis model mice(n=36)were randomly divided into model group,rifampicin group and sodium ferulate group,with 12 mice in each group.Rifampicin group and sodium ferulate group were given 100 mg/kg dose of rifampicin and sodium ferulate respectively,and the mice in the model group were given the same amount of normal saline,once a day,for 6 weeks,and the general characteristics of all mice were observed and recorded;the pathological characteristics of the mice were observed by HE staining at the 2nd,4th,and 6th weeks of administration;MDA detection kit and total SOD activity detection kit were used to determine the level of MDA and SOD activity in lung tissue;Flow cytometry was used to detect the levels of mouse T lymphocyte subsets-CD3T lymphocytes and CD4T lymphocytes;Serum IL-6 and IL-8 levels were detected by enzyme-linked immunosorbent assay;The apoptosis rate of alveolar macrophages was detected by AnnexinV-FITC.Results:The levels of MDA in the lung tissue of the rifampicin group and the sodium ferulate group were lower than those of the model group(P<0.05),and the SOD activity were higher than that of the model group(P<0.05)in the 2nd,4th,and 6th weeks after administration,and there were also significant difference between the rifampicin group and the sodium ferulate group(P<0.05).The ratios of blood CD3T lymphocytes and CD4T lymphocytes in the rifampicin group and sodium ferulate group were significantly higher than those in the model group in the 2nd,4th,and 6th weeks of administration(P<0.05).The sodium group were also higher than the rifampicin group(P<0.05).The serum levels of IL-6 and IL-8 in the rifampicin group and the sodium ferulate group were significantly lower than those in the model group in the 2nd,4th,and 6th weeks after administration(P<0.05),and the sodium fululate group were also lower than the model group.in the rifampicin group(P<0.05).The apoptosis rate of macrophages in BALF in the rifampicin group and sodium ferulate group at the2nd,4th,and 6th weeks of administration were significantly lower than that in the model group(P<0.05).the sodium ferulate group were also significantly lower than the rifampicin group(P<0.05).Conclusion:The application of sodium ferulate in pulmonary tuberculosis model mice can inhibit the expression of inflammatory factors,improve the oxidation status,enhance the immune function of mice,and reduce the apoptosis rate of macrophages in BALF.