生血宝合剂制药过程的废弃沉淀物作为家兔饲料添加剂的可行性研究

Feasibility study on use of waste sediment from pharmaceutical process of Shengxuebao mixture as feed additive for rabbits

试验旨在探究生血宝合剂制药过程中产生的提取液沉淀物(SXBS)用于饲料添加剂的可行性。采用超高效液相色谱串联飞行时间质谱(UPLC-TOF-MS)等技术对SXBS的小分子化学成分和营养成分进行分析,并将SXBS添加在新西兰兔日粮中饲喂60 d,考察SXBS对新西兰兔的生长性能、营养物质表观消化率、屠宰性能、肠道菌群的影响,通过血常规、血生化分析以及组织病理切片观察共同反映SXBS的饲用安全性。结果显示:SXBS含有丰富的碳水化合物、粗纤维、粗蛋白和粗脂肪等营养物质,且含有大量黄酮类、氨基酸类、萜类、多酚类和皂苷类等活性成分。SXBS对新西兰兔生长性能各项指标无明显影响。SXBS可显著调节新西兰兔肠道细菌和真菌的菌群结构,上调亚油酸、丙酸、牛磺酸、半乳糖等与肠道碳水化合物消化吸收相关的代谢功能,增加鲁氏接合酵母丰度。SXBS在60 d内对新西兰兔无明显毒害作用,安全性良好。研究表明,SXBS可作为调节肠道微生态的兔饲料添加剂在家兔养殖中应用。

The experiment was to explore the feasibility of using the extractive liquid sediment(SXBS)produced in pharmaceutical process of Shengxuebao mixture as a feed additive.The research used UPLC-TOF-MS and other technologies to analyze the small molecule chemical composition and nutritional composition of SXBS,and SXBS was added into diet of New Zealand rabbits for 60 d.The effect of SXBS on growth performance,nutrient apparent digestibility,slaughter performance and intestinal flora of New Zealand rabbits were investigated.The feeding safety of SXBS was reflected by blood routine,blood biochemical analysis and histopathological observation.The results showed that SXBS was rich in nutrients such as carbohydrates,crude fiber,crude protein and crude fat,and contained a lot of active ingredients such as flavonoids,amino acids,terpenes,polyphenols and saponins.SXBS had no effect on growth performance of New Zealand rabbits.SXBS could significantly regulate the bacterial and fungal flora structure of New Zealand rabbits’gut,up-regulate linoleic acid,propionic acid,taurine,galactose and other metabolic functions related to the digestion and absorption of intestinal carbohydrates,and increase the abundance of Zygosaccharomyces ruberii.SXBS had no obvious toxic effect on New Zealand rabbits within 60 d,and the safety was good.The study indicates that SXBS can be used in rabbit breeding as a rabbit feed additive for regulating intestinal microecology.