挥发性有机化合物在艰难梭菌感染快速检测中的应用进展

Advances in application of volatile organic compounds in the rapid detection of clostridium difficile infection

艰难梭菌感染(clostridium difficile infection,CDI)是社区和医院较常见的病原菌感染之一,可导致不同程度的腹泻、假膜性肠炎,是全球性的公共健康问题。CDI现有诊断依赖于患者粪便中艰难梭菌(clostridium difficile,CD)相关毒素或毒素编码基因的鉴定,这些传统的细菌学技术具有一定的局限性,可能延误疾病诊疗。研究发现CD有一种特殊的"马粪样"臭味,这种特殊气味来自CD挥发性有机化合物(volatile organic compounds,VOCs)。近年来,越来越多的研究将人体呼气、血液、尿液和粪便中的VOCs作为潜在的非侵入性诊断生物标志物用于感染性疾病和消化道等多种疾病诊断。研究发现,CD的VOCs有助于CDI的快速诊断,其具有潜在的特征性VOCs成分。该文就VOCs及其在CDI快速检测中的应用进行综述。

Clostridium difficile infection(CDI)is a global public health issue and one of the most common pathogenic infections in the community and hospitals,causing varying degrees of diarrhea and pseudomembranous colitis.The current CDI diagnosis is based on the detection of clostridium difficile(CD)associated toxins or toxin encoding genes in patients′stool,but these traditional microbiological techniques have limitations that may delay disease diagnosis.In clinical and research,CD has a distinct odor"horse barn odor",which is caused by the volatile organic compounds(VOCs).VOCs in human breath,blood,urine and feces have recently received increased attention as potential noninvasive diagnostic biomarkers for a wide range of diseases,including infectious diseases and digestive tract disorders.It was discovered that VOCs from CD,with their potential characteristic VOCs,contributed to the rapid diagnosis of CDI.In this paper,we review VOCs and their application in the rapid detection of CDI.