魔芋软腐病病原菌果胶杆菌ZX67菌株全基因组测序及分析

Complete Genome Sequence of Pectobacterium carotovorum Strain ZX67:An Plant Pathogen of Amorphophallus konjac Soft Rot Disease

菌株ZX67是魔芋软腐病病原菌胡萝卜果胶杆菌胡萝卜亚种(Pectobacterium carotovorum subsp.carotovorum),为进一步了解该病原菌的特性,便于更好地研究防治软腐病的方法,对该菌株进行了全基因组测序和分析。该菌株基因组总长度为4,909,724 bp,GC含量为51.27%,包含4,977个编码基因,其序列长度占总基因组的85.25%,预测的非编码RNA主要包括74个tRNA、22个rRNA及100个sRNA,有10个基因岛,6个转座子,预测存在2个前噬菌体,每个前噬菌体的平均长度为26,258 bp,有5个CRISPR序列,每个序列的平均长度为621 bp,并分析了与降解寄主细胞壁相关的裂解酶、与侵染植物相关的5种蛋白分泌系统。以上结果,为进一步了解软腐病病原菌的特性提供了机会,也为研究新的防治方法提供了新的出发点和思路。

Strain ZX67 belonging to Pectobacterium carotovorum subsp.carotovorum is the pathogen of konjac soft rot disease.In order to further understand the characteristics of the pathogen and effectively control and prevent konjac soft rot disease,the whole genome of the strain was sequenced and analyzed.The results showed that its total genome length was 4,909,724 bp and GC-content was 51.27%.It contained 4,977 coding genes,and the sequence accounted for 85.25%of the total genome.The predicted non-coding RNA(ncRNA)mainly included tRNA(74),rRNA(22)and sRNA(100).There were 10 gene islands,and 6 transposons.Two prophages were predicted,the average length of each prophage was 26,258 bp.Five CRISPR sequences were predicted,with the average length of each sequence of 621 bp.In the coding genes,the pectate lyases and other enzymes related to degradation of host plant cells were analyzed.There were five protein secretory systems related to the infected plants.The results provided a new and comprehensive idea for further understanding the characteristics of the pathogen,and studying its prevention and control measures.