小豆WRKY基因家族鉴定及应答锈菌侵染的表达分析

WRKY Gene Family Identification and Expression Analysis of Vigna angularis in Response to Uromyces vignae Infection

为明确小豆WRKY转录因子在抗锈病中的功能,研究采用生物信息学方法,对小豆基因组中WRKY基因家族进行了分析,结果表明,小豆基因组中共包含90个WRKY转录因子(VaWRKYs),其中82个成员分别定位于小豆的11条染色体上。依据WRKY基因家族高度保守的WRKY七肽域和锌指结构对小豆WRKY家族进行分类,发现VaWRKYs包含GroupⅠ(17个)、GroupⅡ(61个)和GroupⅢ(12个)等3个亚家族,进一步的系统发育分析表明,GroupⅡ亚家族还包括Ⅱ-a(6个)、Ⅱ-b(16个)、Ⅱ-c(21个)、Ⅱ-d(7个)和Ⅱ-e(11个)5个亚组。蛋白序列特征分析发现,VaWRKYs成员在七肽域和锌指结构上存在多个氨基酸位点的变异。利用课题组前期获得的抗病小豆品种接种后24和48 h的转录组数据对VaWRKYs成员的表达进行分析,共检测到82个成员不同水平表达,且多达67个成员的表达水平受锈菌侵染的影响,暗示小豆WRKY基因家族在小豆抗锈病过程中起重要的调控作用。

To clarify the function of WRKY transcription factors(TFs) of adzuki bean(Vigna angularis) in response to Uromyces vignae infection,a whole genome identification of WRKY TFs was conducted.The results showed that there were 90 WRKY TFs in V.angularis genome(VaWRKYs),among which 82 members were located on 11 chromosomes,respectively.According to the highly conserved WRKY heptapeptide domain and zinc finger structure,VaWRKYs were divided into 3 subfamilies,including Group Ⅰ(17),Group Ⅱ(61)and Group Ⅲ(12).Phylogenetic analysis showed that the Group Ⅱ subfamily also included 5 subgroups,such as Ⅱ-a(6),Ⅱ-b(16),Ⅱ-c(21),Ⅱ-d(7) and Ⅱ-e(11).Protein sequence analysis showed that there were many variations in heptapeptide domain and zinc finger motif in VaWRKYs.VaWRKYs expression based on the transcriptional data of the resistant cultivar in response to U.vignae at 24 and 48 hours post inoculation(hpi) that obtained by our research group previously were analyzed.Results showed that 82 members of a VaWRKYs were expressed at different levels,of which 67 members were significantly changed during the rust fungal infection.These results indicated that VaWRKYs might play an important regulation role in the resistance of adzuki bean to U.vignae.