摘要
CTAB或者预混试剂盒是当前提取植物基因组DNA的常用方法,提取步骤比较繁琐,提取时间较长且在提取过程中会用到易制毒试剂,具有一定风险性。探索一种适用于植物基因组DNA的绿色不易制毒、操作简单、效率高和耗时短的提取技术,不仅可以节省时间,还能提高效率,避免易制毒试剂对人身和环境的危害。基于上述目的,本研究借鉴其他作物快速高效提取基因组DNA的方法,基于NaOH裂解法建立了一个简便快速提取大豆基因组DNA的技术方法。该技术方法能满足常规分子辅助育种的高通量样品基因组PCR检测,具有一定的利用价值和应用前景。
Usually,CTAB method or kit and other methods are commonly used to extract plant genomic DNA,but the extraction procedures are often cumbersome,the extraction time is long,and poisonable chemicals will be often used in the extraction process,which has certain risks to our health and surrounding environment.Exploring a green extraction technology suitable for genomic DNA that is not poisonable,simple to operate,high efficiency and short in time can not only save experimental time,but also improve experimental efficiency.The current study uses NaOH to carry out a simple and rapid extraction of soybean DNA.This technology meets the conventional PCR detection and has certain utilization value and application prospects.
作者
杜艳伟
张煦
李佳男
姜振峰
DU Yan-wei;ZHANG Xu;LI Jia-nan;JIANG Zhen-feng(Northeast Agricultural University/Key Laboratory of Soybean Biology in Chinese Ministry of Education/Key Laboratory of Soybean Biology and Genetics Breeding of Chinese Agriculture Ministry,Harbin 150030,China;North Corporation of Sinograin,Nenjiang 161400,China)
出处
《黑龙江农业科学》
2022年第12期15-19,共5页
Heilongjiang Agricultural Sciences
基金
国家自然科学基金项目(31571693,32172072)
黑龙江省自然科学基金项目(LH2021C025)。
