糖尿病肾病患者中血尿酸、IL-8和TNF-α水平的变化

Changes of serum uric acid IL-8 and TNF-αin patients with diabetic nephropathy

目的:分析糖尿病肾病(diabetic nephropathy,DN)与血尿酸(serum uric acid,SUA)、白介素-8(interleukin,IL-8)及肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)之间的关系。方法:选择2020年9月-2021年6月收治的2型糖尿病患者130例,依据患者尿白蛋白排泄率(UAER)或24 h尿蛋白定量将病例分为3组,单纯2型糖尿病组(UAER<20μg/min或24 h尿蛋白定量<30 mg)49例,微量蛋白尿组(UAER 20~200μg/min或24 h尿蛋白定量30~300 mg)37例,大量蛋白尿(UAER>200μg/min或24 h尿蛋白定量>500 mg)44例,另收集同期44例健康体检者作为对照组。采用酶联免疫吸附法(ELISA)检测各组血IL-8、TNF-α水平并进行对比分析。结果:与对照组相比,三组患者的血尿酸水平逐渐升高(P<0.05),且血IL-8与TNF-α水平随着尿白蛋白量的增加而明显升高(P<0.05);患者的血尿酸水平及炎性因子IL-8与TNF-α的表达具有正相关性(P<0.05)。结论:2型糖尿病患者的血尿酸水平与尿白蛋白水平升高呈相关性,且高尿酸血症相关的炎症因子IL-8、TNF-α与尿白蛋白升高也呈明显相关性,考虑2型糖尿病肾病由多种因素所致,其中炎症反应可能起着重要的作用。

Objective:to analyze the association between diabetic nephropathy and serum uric acid,interleukin-8 and tumor necrosis factorαRelationship between the three.Methods:the analysis subjects a total of 130 type 2 diabetic patients admitted to our hospital from September 2020 to June 2021 were selected,and the selected cases were divided according to the urinary albumin excretion rate(UAER)or(24h urinary protein quantification):49 in type 2 diabetes alone group(UAER<20μg/min or 24 h urinary protein quantification<30 mg),37 in microalbuminuria group(UAER 20-200μg/min or 24 h urinary protein quantification 30-300mg),And 44 cases with massive proteinuria(UAER>200μg/min or 24 h urinary protein quantification>500 mg),another 44 healthy individuals in the same period were collected and classified as the control group.Interleukin-8(IL-8)and TNF were measured by enzyme-linked immunosorbent assay(ELISA)in all patientsαLevels.Serum uric acid,interleukin-8,and TNF were measured in the four groups according to different grades of urinary albumin excretionαA comparative analysis was performed.Results:compared with healthy controls,patients with type 2 diabetes alone and those with microalbuminuria had increased serum uric acid levels,whereas those with macroalbuminuria had significantly higher serum uric acid levels(P<0.05).Interleukin-8 and TNF in four cohorts-αLevels were significantly higher with increasing urinary albumin volume(P<0.05).At the same time,the serum uric acid levels and the expression of inflammatory factors in the four groups of patients had a further proportional Association(P<0.01).Conclusion:there was a significant correlation between serum uric acid level and urinary albumin level in patients with type 2 diabetes.And hyperuricemia related inflammatory factors IL-8 and TNF-αIt is also related to the elevation of urinary albumin.Therefore,the occurrence of type 2 diabetic nephropathy is caused by a variety of factors.Inflammation and oxidative stress play an important role.