胃癌组织中miR-143-3p、LASP1表达与临床病理特征和预后的关系

Relationships between expression of miR-143-3p,LASP1 and clinicopathological features and prognosis in gastric cancer tissues

目的探讨胃癌组织中微小RNA-143-3p(miR-143-3p)、LIM和Src同源结构域3蛋白1(LASP1)表达与临床病理特征和预后的关系。方法选取113例胃癌患者,采用qPCR法检测胃癌组织和癌旁组织中miR-143-3p、LASP1 mRNA表达。采用Spearman相关性分析胃癌组织中miR-143-3p与LASP1 mRNA表达的关系,比较不同临床病理特征患者miR-143-3p、LASP1 mRNA表达。对患者进行随访,采用Kaplan-Meier法绘制胃癌组织中不同miR-143-3p、LASP1 mRNA表达患者生存曲线。结果与癌旁组织比较,胃癌组织中miR-143-3p表达下调,LASP1 mRNA表达上调(P均<0.05)。Spearman相关性分析显示,胃癌组织中miR-143-3p与LASP1 mRNA表达呈负相关(r=-0.810,P<0.01),二者与TNM分期和淋巴结转移有关(P均<0.05)。随访3年,113例胃癌总生存率为69.03%(78/113)。Kaplan-Meier生存曲线分析显示,miR-143-3p高表达者3年总生存率高于miR-143-3p低表达者,LASP1 mRNA高表达者3年总生存率低于LASP1 mRNA低表达者(P均<0.05)。结论胃癌组织中miR-143-3p低表达,LASP1 mRNA高表达,与TNM分期、淋巴结转移和预后有关,二者可能成为胃癌预后的评估指标。

Objective To investigate the relationships between the expression of microRNA-143-3p(miR-143-3p),LIM and Src homology 3 protein 1(LASP1)in the gastric cancer tissues and their clinicopathological characteristics and prognosis.Methods Totally 113 gastric cancer patients were selected and miR-143-3p and LASP1 mRNA expression in gastric cancer tissues and paraneoplastic tissues were detected by qPCR.We compared miR-143-3p and LASP1 mRNA ex⁃pression in patients with different clinicopathological features.Patients were followed up and survival curves of patients with miR-143-3p and LASP1 mRNA expression in different gastric cancer tissues were plotted using the Kaplan-Meier method.Results Compared with paracarcinoma tissues,the miR-143-3p expression was down-regulated and LASP1 mRNA expression was up-regulated in the gastric cancer tissues(both P<0.05).Spearman correlation analysis showed that miR-143-3p was negatively correlated with LASP1 mRNA expression in the gastric cancer tissues(r=-0.810,P<0.01),and both were associated with TNM stage and lymph node metastasis(both P<0.05).At 3 years of follow-up,the overall survival rate of 113 cases of gastric cancer was 69.03%(78/113).Kaplan-Meier survival curve analysis showed that the overall survival rate of the high miR-143-3p expression group was higher than that of the low miR-143-3p expres⁃sion group,and the overall survival rate of the high LASP1 mRNA expression group was lower than that of the low LASP1 mRNA expression group(both P<0.05).Conclusion Low expression of miR-143-3p and high expression of LASP1 mRNA in gastric cancer tissues are associated with TNM stage,lymph node metastasis,and prognosis,and both of them may be the indicators for prognostic assessment of gastric cancer.