应用“启枢散精化浊”法改善糖尿病GK大鼠胰岛微循环对血糖波动及肠道GLP-1分泌水平的影响

The Effects of "Dredging Hub, Divergenting Serum and Refinding Turbid" to Improve the Pancreatic Islet Microcirculation on Improving Blood Glucose Fluctuation and Intestinal GLP-1 Secretion in Diabetes Mellitus GK Rats

目的 探讨“启枢散精化浊”法即应用我院院内制剂三生调脂舒改善糖尿病GK大鼠胰岛微循环对血糖波动及肠道胰高血糖素样肽1(glucagon-like peptide-1,GLP-1)分泌水平的影响。方法 建立由高脂饲料喂养的糖尿病大血管病变大鼠模型。实验分4组,空白对照组(正常Wistar大鼠组),模型组,三生调脂舒组,阳性对照组。于灌胃后每周1次测1日内8:00、10:00、14:00、16:00、18:00 5次血糖水平,并用血糖稳定性指标(MBG、SDBG、LAGE)量化各组血糖稳定性;检测血清GLP-1、血清胰岛素水平。结果 三生调脂舒组各状况优于模型组,可减少大鼠的血糖波动,升高血清GLP-1水平;胰岛素(INS)与模型组比,无统计学意义;GLP-1水平较模型组有明显增加(P<0.01)。结论 应用“启枢散精化浊”法治疗糖尿病GK大鼠,可促进GLP-1的分泌,降低血糖,减少血糖的波动,保持血糖的稳态,减轻模型GK大鼠血管内皮损伤,改善糖尿病大血管的病变,从而起到延缓糖尿病进展的作用。

Objective To explore the effect of "Dredging hub, divergenting serum and refinding turbid" method,namely the application of Sansheng Tiaozhishu to improve the blood glucose fluctuation and intestinal glucagon-like peptide-1(GLP-1) secretion level in diabetic GK rats. Methods A rat model of diabetic macroangiopathy was established by high-fat diets. The rats were divided into 4 groups: blank control group, model group, Sansheng Tiaozhishu group and positive control group. After intragastric administration, their blood glucose levels were measured once a week at 8:00, 10:00, 14:00, 16:00 and 18:00, and their blood glucose stability indexes(MBG, SDBG and LAGE) were used to quantify the blood glucose stability of each group. The levels of serum GLP-1, serum insulin and glucagon were measured. Results The condition of Sansheng Tiaozhishu group was better than that of the model group, which could reduce the fluctuation of blood glucose and increase the level of serum GLP-1. Compared with the model group, insulin(INS) had no statistical significance but GLP-1 was significantly higher than that of the model group( P <0.01). Conclusion The application of the method of "Dredging hub, divergenting serum and refinding turbid" in the treatment of diabetic GK rats with the Sansheng Tiaozhishu in our hospital can promote the secretion of GLP-1, reduce blood sugar and the fluctuation of blood sugar, so as to maintain the homeostasis of blood glucose, alleviate the injury of vascular endothelium in model GK rats, and improve the pathological changes of the large blood vessels of the disease, thereby slowing the progression of diabetes.