DNA甲基化测量技术及准确性评估研究进展

Research Progress of DNA Methylation Measurement Technology and Accuracy Evaluation

DNA甲基化是人们深入研究的最重要的表观遗传机制。基于测序的DNA甲基化分析为描绘比较完整的DNA CpG图谱奠定了基础。在过去的几十年里,大量DNA甲基化测量技术的涌现使基因组甲基化分析研究得到了极大的发展。但由于不同实验室设备及人员操作等方面存在很大差异,很难进行实验室间的测量结果的比较。同时由于缺乏评估基因组甲基化的标准分析流程和标准物质,尚不明确目前甲基化特异性是否如通常识别序列所暗示的那样精确。因此,对DNA甲基化测量技术的发展以及面临的问题展开了讨论,阐述了DNA甲基化测量准确性评估和标准物质的重要性。

DNA methylation is the most important epigenetic mechanism that has been deeply studied.DNA methylation analysis based on sequencing has laid the foundation for describing a relatively complete DNA CpG map.In the past few decades,the emergence of a large number of DNA methylation measurement technologies has greatly improved the research of genome methylation analysis.However,due to the great differences in equipment and personnel operations of different laboratories,it is difficult to compare the measurement results between laboratories.At the same time,due to the lack of standard analysis procedures and reference materials for evaluating genome methylation,it is not clear whether the current methylation specificity is as accurate as the usual recognition sequence implies.Therefore,the development and problems of DNA methylation measurement technology are discussed,and the importance of DNA methylation measurement accuracy evaluation and reference materials is proposed.