慢性牙周炎患者血清CGRP、PGE2、CCL20与牙周临床指标和Th17/Treg失衡的相关性分析

Correlation Analysis of Serum CGRP,PGE2,CCL20 and Periodontal Clinical Indicators and Th17/Treg Imbalance in Patients with Chronic Periodontitis

目的:探讨慢性牙周炎(CP)患者血清降钙素基因相关肽(CGRP)、前列腺素E2(PGE_(2))、C-C基序趋化因子配体20(CCL20)与牙周临床指标和辅助性T细胞17/调节性T细胞(Th17/Treg)失衡的相关性。方法:选取2018年3月~2022年1月本院收治的92例CP患者,根据病情严重程度分为轻度组28例、中度组33例、重度组31例。检查患者牙周临床指标,采用酶联免疫吸附法检测血清CGRP、PGE_(2)、CCL20水平,流式细胞术检测外周血Th17、Treg百分比并计算Th17/Treg。比较3组血清CGRP、PGE_(2)、CCL20水平和牙周临床指标及外周血Th17、Treg、Th17/Treg,采用Pearson相关系数分析CP患者血清CGRP、PGE_(2)、CCL20水平与牙周临床指标和Th17/Treg的相关性。结果:轻度组、中度组、重度组血清CGRP水平和外周血Treg比例依次降低,血清PGE_(2)、CCL20水平和探诊深度(PD)、出血指数(BI)、附着丧失(AL)、菌斑指数(PLI)及外周血Th17比例、Th17/Treg依次升高(P<0.05)。Pearson相关系数显示,CP患者血清CGRP水平与PD、BI、AL、PLI、Th17/Treg呈负相关,PGE_(2)、CCL20水平与PD、BI、AL、PLI、Th17/Treg呈正相关(P<0.05)。结论:血清CGRP、PGE_(2)、CCL20水平与CP患者牙周临床指标和Th17/Treg失衡密切相关,可能是CP发生发展的关键因子。

Objective:To investigate the correlation between serum calcitonin gene-related peptide(CGRP),prostaglandin E2(PGE_(2))and C-C motif chemokine ligand 20(CCL20)and periodontal clinical indicators and helper T helper cell 17/regulatory T cell(Th17/Treg)imbalance in patients with chronic periodontitis(CP).Methods:92 patients with CP who were admitted to our hospital from March 2018 to January 2022 were selected,and they were divided into mild group with 28 cases,mild group with 33 cases and sild group with 31 cases according to the different severity.The periodontal clinical indicators of patients were examined.The levels of serum CGRP,PGE_(2) and CCL20 were detected by enzyme-linked immunosorbent assay.The percentages of peripheral blood Th17 and Treg were detected by flow cytometry and Th17/Treg was calculated.The levels of serum CGRP,PGE_(2),CCL20,periodontal clinical indicators and peripheral blood Th17,Treg,Th17/Treg were compared among the three groups.Pearson correlation coefficient was used to analyze the correlation between the levels of serum CGRP,PGE_(2),CCL20 and periodontal clinical indicators and Th17/Treg in patients with CP.Results:The levels of serum CGRP and the ratio of peripheral blood Treg in mild group,moderate group and severe group decreased in turn,while the levels of serum PGE_(2) ,CCL20,probing depth(PD),bleed index(BI),attachment loss(AL),plaque index(PLI),the ratio of peripheral blood Th17 and Th17/Treg increased in turn(P<0.05).Pearson correlation coefficient showed that the level of serum CGRP was negatively correlated with PD,BI,AL,PLI and Th17/Treg in patients with CP,and the levels of PGE_(2) and CCL20 were positively correlated with PD,BI,AL,PLI and Th17/Treg(P<0.05).Conclusion:The levels of serum CGRP,PGE_(2) and CCL20 are closely related to periodontal clinical indicators and Th17/Treg imbalance in patients with CP,and which may be the key factors for the occurrence and development of CP.