双目峰口岸鼠类携带汉坦病毒情况分析

Analysis on the Situation of Hantavirus Carried by Rodents at Shuangmufeng Port

掌握双目峰口岸鼠类分布以及汉坦病毒流行情况,以加强该口岸地区汉坦病毒的防控。在双目峰口岸地区采用夹夜法捕鼠,取肺组织提取RNA,采用特异性汉坦引物(M段)进行RT-PCR检测汉坦病毒,测序结果经NCBI-blast比对后,使用MEGA 7.0构建部分M段汉坦病毒系统发育树。共采集了97只鼠,形态学鉴定结果为红背䶄、棕背䶄等共5种。检测出的汉坦病毒均为汉城型,带毒率为22.68%。大林姬鼠带毒率最高为29.42%,其次为红背䶄和棕背䶄。基于邻接法(NJ)构建的M段汉坦病毒系统发育树显示,22个SEO型汉坦病毒测序样本均与朝鲜汉坦病毒DPRK08聚类在一起。本次的监测结果显示棕背䶄、红背䶄、大林姬鼠均为该地区的优势种,汉坦病毒病原体检出率较高,以上结果均与以往调查结果相同。

This study is to grasp the distribution of rodents and the prevalence of Hantavirus at Shuangmufeng Port,so as to strengthen the prevention and control of Hantavirus in the port area.In the Shuangmufeng Port area,the mice were trapped by nighttime method.The lung tissue of the samples was taken to extract RNA,RT-PCR detection of Hantavirus was carried out with specific hantaan primers(M segment),and the positive samples were isolated and purified,after the sequencing results were compared by NCBI-blast.MEGA7.0 was used to construct a partial M-segment Hantavirus phylogenetic tree(Neighbor-Joining method).A total of 97 mice of 5 species were collected,including Clethrionomys rutilus,Clethrionomys rufocanus,etc.All of which were Seoul Hantavirus(SEO),with a virus-carrying rate of 22.68%.The highest poisoning rate was 29.42%,followed by Clethrionomys rutilus and Clethrionomys rufocanus.The M-segment Hantavirus phylogenetic tree constructed based on the neighbor joining method(NJ)showed that all 22 SEO-type Hantavirus sequencing samples were clustered with Korean Hantavirus DPRK08.From this background monitoring,it was shown that the Clethrionomys rufocanus,the Clethrionomys rutilus,and the Apodemus peninsulae were the dominant species in the forest area,and the detection rate of the Hantavirus pathogen was relatively high.