地衣芽孢杆菌ZSZ6降解羽毛粉产氨基酸的静置处理工艺优化

Process Optimization of Amino Acid Production from Feather Powder by Bacillus licheniformis ZSZ6

为提高羽毛角蛋白的资源化利用率,初步探索了静置处理对地衣芽孢杆菌ZSZ6降解羽毛粉产氨基酸的影响,并通过单因素试验考察静置处理前的好氧培养时间、静置温度、还原剂预处理、添加金属离子等对羽毛降解产氨基酸效果的影响,结果表明:在250 mL的三角瓶中,经1%Na_(2)SO_(3)预处理1 d的羽毛粉培养基,在37℃、180 r·min^(-1)好氧发酵2 d后,添加0.1%MnSO_(4)并于55℃条件下静置处理,在处理的第6天,产物中的游离氨基酸含量可达35.41 g·L^(-1),转化率约35%。综上,静置处理是一种能够有效提高地衣芽孢杆菌ZSZ6降解羽毛粉产氨基酸的方法。

Keratin,as the main component of feathers,is a kind of fibrous structural protein which is difficult to degrade in nature.A large number of feather wastes are not fully utilized in China every year.In order to improve the resource utilization rate,this study explored the effect of static treatment on the degradation of feather powder into amino acids by Bacillus licheniformis ZSZ6.The effects of aerobic culture time,static temperature,reductant pretreatment and the addition of metal ions on the production of amino acids were also investigated through single factor test before the static treatment.Finally,the results showed that 100 mL feather culture medium pretreated with 1% Na_(2)SO_(3),aerobic cultured 180 r·min^(-1) at 37℃ for 2 days,then added with 0.1% MnSO_(4) and treated at 55℃ in a 250 ml triangular flask might reach the maxim yield.On the 6th day,the content of amino acids in the product could reach 35.41 g·L^(-1),and the conversion rate was about 35%.In conclusion,static treatment was an effective method to improve the yield of feather powder into amino acids degraded by Bacillus licheniformis ZSZ6.