外源菌降解褐煤产气特征研究

Study on Gas Production Characteristics of Lignite Degraded by Exogenous Bacteria

利用外源菌来增产煤层气是生物促产煤层气领域研究热点,以寺河煤层水富集培养菌群为外源菌,内蒙胜利、河南义马、云南昭通褐煤作为产气底物,分析3种褐煤的产气特征。对内蒙胜利褐煤产气组采用高通量测序,分析微生物菌群结构,判断可能的产气途径。研究结果表明:70 d的产气周期内,内蒙有2个产甲烷高峰,云南和义马均有1个产气高峰。最大产甲烷量分别为81.01、22、31.54 mL,产甲烷率分别为8.012、2.111、3.065 mL/g。产气周期内,氢气逐渐减少,二氧化碳与甲烷含量呈现负相关,产气途径偏向于H_(2)/CO_(2)还原途径。内蒙褐煤产气微生物群落结构中存在的W22,Citrobacter,Syntrophomonas,Desulfovibrio,Methanomicrobiales等菌群,也说明了产甲烷类型可能以H_(2)/CO_(2)还原途径为主。

Using exogenous bacteria to increase coalbed methane production is a research hotspot in the field of biological promotion of coalbed methane production.Taking Sihe coalbed water enrichment and culture bacteria as exogenous bacteria,Inner Mongolia Shengli,Henan Yima and Yunnan Zhaotong lignite as gas production substrates,analyzes the gas production characteristics of three kinds of lignite.High throughput sequencing was used for Shengli lignite gas production group in Inner Mongolia to analyze the microbial flora structure and judge the possible gas production pathway.The results show that there are two methane production peaks in Inner Mongolia and one in Yunnan and Yima during the 70 day gas production cycle.The maximum methane production was 81.01 mL,22 mL and 31.54 mL respectively,and the methane production rates were 8.012 mL/g,2.111 mL/g and 3.065 mL/g respectively.During the gas production cycle,hydrogen gradually decreases,carbon dioxide and methane content show a negative correlation,and the gas production path is inclined to H_(2)/CO_(2)reduction path.W22,Citrobacter,Syntrophomonas,Desulfovibrio,Methanomicrobiales and other flora in the microbial community structure of lignite gas production in Inner Mongolia also indicate that the type of methane production may be dominated by H_(2)/CO_(2)reduction pathway.