超声辅助吐温80提取发芽小米GABA和多酚的工艺优化

Optimization of Ultrasound-assisted Tween 80 Extraction of GABA and Polyphenols from Germinated Millet

采用超声辅助吐温80优化发芽小米γ-氨基丁酸(gamma-amino butyric acid,GABA)和多酚提取工艺。以GABA和多酚得率为考察指标,通过单因素试验探究吐温80质量分数、超声温度、超声时间、料液比、超声功率对GABA得率的影响,设计正交试验优化GABA提取工艺;通过单因素试验探究吐温80添加量、超声温度、超声时间、料液比4个因素对多酚得率的影响,以响应面试验设计优化多酚提取工艺。结果表明:超声辅助吐温80提取GABA的最佳工艺条件为吐温80质量分数6%、超声温度35℃、超声时间25 min、料液比1∶18(g/mL)、超声功率126 W,此条件下GABA得率为4.70 mg/g;提取多酚的最佳工艺条件为吐温80质量分数9%、超声温度42℃、超声时间19 min、料液比1∶12.5(g/mL),此条件下多酚得率为1.99 mg/g。

Ultrasound-assisted Tween 80 was used to optimize the extraction of gamma-amino butyric acid(GABA)and polyphenols from germinated millet.Taking GABA and polyphenol yield as the inspection index,the influence of Tween 80 mass fraction,ultrasonic temperature and time,solid-liquid ratio,and ultrasonic power on the GABA yield were investigated using the single factor test,and an orthogonal test was designed to optimize the extraction process of GABA.The effects of Tween 80 mass fraction,ultrasonic temperature and time,and solid-liquid ratio on the yield of polyphenols were also studied using the single factor test,and the extraction process of polyphenols was optimized through response surface design.The results showed that the optimum technological parameters of ultrasound-assisted Tween 80 to extract of GABA were:a Tween 80 mass fraction of 6%,ultrasonic temperature of 35℃,ultrasonic time of 25 min,solid-liquid ratio of 1∶18(g/mL),and ultrasonic power of 126 W.Under these conditions,the final GABA yield was 4.70 mg/g.The optimum technological parameters for extracting polyphenols were:a Tween 80 mass fraction of 9%,ultrasonic temperature of 42℃,ultrasonic time of 19 min,and solid-liquid ratio of 1∶12.5(g/mL).Under these conditions,the final polyphenol yield was 1.99 mg/g.