口虾蛄总生物碱对人鼻咽癌CNE-2Z细胞周期与凋亡的影响

The effects of the total alkaloids of Squilla oratoria on proliferation and apoptosis of Nasopharyngeal Carcinoma cell line CNE-2Z

目的提取口虾蛄总生物碱并观察其对人鼻咽癌CNE-2Z细胞增殖、凋亡的影响。方法酸提碱沉法处理口虾蛄,沉淀部分以乙酸乙酯萃取;不同浓度的口虾蛄总生物碱处理CNE-2Z细胞,CCK-8法、平板克隆形成实验检测细胞增殖能力的影响;流式细胞术检测细胞周期分布的影响;蛋白免疫印迹实验检测Caspase8蛋白表达的变化。结果口虾蛄总生物碱能明显抑制CNE-2Z细胞的增殖,降低克隆形成能力,使细胞周期阻滞于S期;Caspase8蛋白表达水平随着口虾蛄总生物碱浓度增高而降低,有一定的剂量依赖。结论口虾蛄总生物碱抗CNE-2Z细胞的作用可能通过抑制细胞增殖、诱导细胞周期阻滞及细胞凋亡等发生。

Objective To extract the total alkaloid of Squilla oratoria and observe its effects on the proliferation and apoptosis of human nasopharyngeal carcinoma line CNE-2Z.Methods Acid extraction and alkaline deposition,and ethyl acetate extraction were used in the extraction and separation of Squilla oratoria,CNE-2Z cells were treated with various concentrations of total alkaloid,then the ability of cell proliferation was detected by CCK-8 assay and colony-forming assay,and the changes of cell cycle distributions were analyzed by flow cytometry.The expression of caspase-8 protein was detected by Western blotting.Results The total alkaloid of Squilla oratoria effectively inhibited the proliferation and colony formation of CNE-2Z cells in certain dose-dependent way,which was related to its induction of cell cycle arrest at S phase.The expression of caspase-8 was decreased after treated with total alkaloid.Conclusion The effect of total alkaloids of Squilla oratoria on CNE-2Z cells can be induced by inhibiting cell proliferation,inducing cell cycle arrest and inducing apoptosis.