强直性脊柱炎患者Th17/Treg相关细胞因子及miR-155与脊柱活动度的关系

The relationship between Th17/Treg-related cytokines,miR-155 and spinal mobility in patients with ankylosing spondylitis

目的探讨强直性脊柱炎患者辅助性T细胞17(Th17)/调节性T细胞(Treg)相关细胞因子、微小核糖核酸-155(miR-155)与脊柱活动度的关系。方法回顾性分析2018年1月~2021年3月我院收治的强直性脊柱炎患者116例为观察组,根据强直性脊柱炎疾病活动指数(BASDAI)分为活动期(BASDAI≥4,n=52)、稳定期(BASDAI<4,n=64);另选取同期于我院进行健康体检的健康志愿者97例为对照组。对比各组Th17/Treg相关细胞因子、miR-155表达水平;对比活动期和稳定期的脊柱活动度Bath AS测量指数(BASMI),分析强直性脊柱炎患者Th17/Treg相关细胞因子、miR-155与脊柱活动度的相关性。结果观察组IL-17、IL-23、miR-155表达水平明显高于对照组(P<0.05)。活动期IL-17、IL-23、miR-155表达水平明显高于稳定期(P<0.05)。活动期强直性脊柱炎患者BASMI评分明显高于稳定期(P<0.05)。经Pearson相关系数分析显示,强直性脊柱炎患者IL-17、IL-23、miR-155表达水平与BASMI评分呈正相关,IL-10、TGF-β表达水平与BASMI评分呈负相关(P<0.05)。结论强直性脊柱炎患者高表达miR-155、Th17相关细胞因子、低表达Treg相关细胞因子,Th17/Treg呈失衡状态,且Th17/Treg相关细胞因子、miR-155与强直性脊柱炎患者脊柱活动度具有一定的相关性,可作为临床评估病情的辅助检查。

Objective To investigate the relationship between helper T cell 17(Th17)/regulatory T cell(Treg)related cytokines,miR-155(miR-155)and spinal activity in patients with ankylosing spondylitis.Methods A total of 116 patients with ankylosing spondylitis admitted to our hospital from January 2018 to March 2021 were enrolled as observation group,and parameter N was divided into active stage(BASDAI≥4,N=52)and stable stage(BASDAI<4,N=64)according to BASDAI disease activity index(BASDAI).Another 97 healthy volunteers who underwent physical examination in our hospital during the same period were selected as control group.The expression levels of Th17/Treg related cytokines and miR-155 in each group were compared.The Bath AS index(BASMI)of spinal activity was compared between active and stable periods,and the correlation of Th17/Treg related cytokines,miR-155 and spinal activity was analyzed in ankylosing spondylitis patients.Results The expression levels of IL-17,IL-23,and miR-155 in the study group were significantly higher than those in the control group(P<0.05).The expression levels of IL-17,IL-23,and miR-155 in the active phase were significantly higher than those in the stable phase(P<0.05).The BASMI score of patients with active ankylosing spondylitis was significantly higher than that in the stable phase(P<0.05);④Pearson correlation coefficient analysis showed that the expression levels of IL-17,IL-23,miR-155 in patients with ankylosing spondylitis were positively correlated with the BASMI score,and the expression levels of IL-10 and TGF-βwere negatively correlated with the BASMI score(P<0.05).Conclusion Patients with ankylosing spondylitis have high expression of miR-155 and Th17-related cytokines,low expression of Treg-related cytokines,Th17/Treg imbalance,and Th17/Treg-related cytokines and miR-155 have a certain correlation with the patient's spinal mobility,which can be used as an auxiliary examination for clinical assessment of the condition.