土家族药物豆板还阳的HPLC指纹图谱研究

Study on HPLC Fingerprint of Tujia Ethnic Medicine Douban Huanyang

目的:建立土家族药物豆板还阳的HPLC指纹图谱特征,为豆板还阳的真伪鉴别和质量控制提供参考。方法:采用Inertsustain C_(18)色谱柱(4.6 mm×150 mm,5μm),检测波长为332 nm,柱温30℃,乙腈(A)-0.1%磷酸水(B)为流动相,梯度洗脱:0~25 min,15%A;25~55 min,15%→52%A,流速1.0 mL/min,进样量10μL。测定8批豆板还阳的指纹图谱,筛选其共有模式,标定共有指纹峰,采用中药色谱指纹图谱相似度评价系统(2012A版)软件计算相似度,以石吊兰素对照品指认色谱峰。结果:建立了土家族药物豆板还阳的HPLC指纹图谱,对8批样品的相似度进行了比较,标定了16个共有峰,并指认了16号峰为石吊兰素。结论:不同产地土家族药物豆板还阳主成分基本相同,但各组分含量的差别较大;本研究建立的方法简便快捷,重复性好,特征性强,可为豆板还阳的真伪鉴别、资源开发利用和质量控制提供参考。

Objective:To provide the reference for true and false identification of Douban Huanyang(Lysionotus pauciflorus)and quality control by establishing the characteristics of HPLC fingerprints of the medicine.Methods:Inertsustain C_(18)chromatographic column(4.6 mm×150 mm,5μm)was adopted,detection wavelength was 332 nm,column temperature 30℃,acetonitrile(A)-0.1%phosphate water(B)as mobile phase,gradient elution:0~25 min,15%A;25~55 min,15%→52%A,flow velocity:1.0 mL/min,sample size was 10μL.The fingerprint patterns of eight batches of Douban Huanyang were determined,to screen the common patterns,and calibrate the common fingerprint peaks,similarity evaluation system of chromatographic fingerprint of traditional Chinese medicine(2012A edition)was adopted to calculate the similarity,chromatographic peaks were identified with the reference substance of lysionotin.Results:HPLC fingerprint of Douban Huanyang has been established,and the similarity of eight batches of the samples was compared,and the common fingerprint peaks were calibrated,meanwhile,peak No.16 has been identified as lysionotin.Conclusion:The principal components of Douban Huanyan from different producing areas are basically the same,while there are disparities between the contents of the components;the method established in the study,simple and convenient,rapid,reproducible and strong features,could provide the reference for true and false identification,resource exploitation and utilization,and quality control of Douban Huanyang.