摘要
文章综述了以分子印迹技术及高效液相色谱—质谱法为原理的快速检测蜂王浆中甾体激素类成分及其含量的方法。重点介绍了液液萃取法、固相萃取法、QuECHERS等前处理方法以及高效液相色谱—质谱法(HPLC-MS)、气相色谱—质谱法(GC-MS)、放射免疫测定法、酶联免疫吸附法等分析方法的研究进展,分析比较了各种前处理方法及分析方法的优缺点,并对分子印迹技术与高效液相色谱—质谱法联用检测蜂王浆中甾体激素的可行性进行了展望。
The rapid determination of steroidal hormones and their contents in royal jelly by molecular imprinting and high performance liquid chromatography-mass spectrometry(HPLC-MS)was reviewed.The research progress of liquid-liquid extraction,solid phase extraction,QuECHERS and other pretreatment methods,as well as HPLC-MS,gas chromatography-mass spectrometry(GC-MS),radioimmunoassay and enzyme-linked immunosorbent assay were introduced.The advantages and disadvantages of various pretreatment methods and analytical methods were analyzed and compared.Finally,the feasibility of using molecular imprinting technology and HPLC-MS to detect steroid hormones in royal jelly was prospected.
作者
李铮
杨静
焦丽丽
李慧
吴巍
LI Zheng;YANG Jing;JIAO Li-li;LI Hui;WU Wei(Changchun University of Chinese Medicine,Changchun,Jilin 130000,China;Tonghua Jitong Pharmaceutical Co.,Ltd.,Tonghua,Jilin 134000,China)
出处
《食品与机械》
北大核心
2022年第11期206-211,共6页
Food and Machinery
基金
吉林省科技发展项目(编号:20200708042YY)。
关键词
蜂王浆
甾体激素
前处理方法
分析方法
royal jelly
steroid hormones
pre-treatment method
analytical method
