细粒棘球绦虫抗原B对免疫性血小板减少症小鼠模型免疫调节作用的初步研究

Preliminary study of the immunomodulatory effect of Echinococcus granulosus sensu stricto antigen B on Immune Thrombocytopenia mouse model

目的探讨细粒棘球绦虫(Echinococcus granulosus sensu stricto,E.granulosus s.s.)囊液自然抗原B(natural antigen B,nAgB)对免疫性血小板减少症(Immune thrombocytopenia,ITP)的调节作用。方法50只8~10周龄Balb/C雌性小鼠随机分5组,每组10只进行腹腔注射实验:I组:PBS处理组即空白对照组;II组:大鼠抗小鼠CD41单克隆抗体(anti-CD41 Ab)免疫组即ITP模型组;III组:nAgB对照组;IV组:nAgB连续免疫5 d后给予anti-CD41 Ab免疫组即nAgB+ITP组;V组:在anti-CD41 Ab免疫建立和维持ITP模型基础上,注射nAgB组即ITP+nAgB组。观察各组血小板(PLT)指标的变化。结果小鼠干预前血PLT计数均为(749.86±84.61)×10^(9)/L。II组和V组给予anti-CD41 Ab 2 d后分别与干预前比较差异均有统计学意义(t=31.299,t=20.955,均P<0.001),ITP建模成功。II组建模成功后与IV组PLT计数比较差异有统计学意义(t=7.493,P<0.001)。II组建模成功后第2 d与V组PLT计数比较差异有统计学意义(t=10.829,P<0.001)。II组建模成功后平均血小板体积(MPV)数值和血小板压积(PCT)数值分别与I组MPV数值和PCT数值比较差异有统计学意义(t=9.615,P<0.001;t=28.698,P<0.001)。结论AgB对免疫损伤所致ITP具有预防及治疗作用。

Objective The aim of the study was to investigate the regulatory effect of Echinococcus granulosus sensu stricto(E.granulosus s.s.)cyst liquid natural antigen B(nAgB)on Immune Thrombocytopenia(ITP).Methods Fifty aged 8-10 weeks female BALB/C mice were randomly divided into five groups.The mice of each group were treated as follows:Group I,treated with PBS,namely,blank control group;Group II,treated with anti-CD41 monoclonal antibody(anti-CD41 Ab)only,namely,ITP mouse model group;Group III,treated with nAgB only,namely,nAgB control group;Group IV,treated with nAgB and anti-CD41 Ab successively,namely nAgB+ITP group;Group V,on the basis of establishing and maintaining ITP model of anti-CD41 Ab immunization,then injection nAgB group,namely ITP+nAgB group.Platelet index was compared between different groups.Results The platelet(PLT)count of all mice before intervention was(749.8684.61)10^(9)/L.The PLT count of group II and group V were significantly lower than the mice before intervention(t=31.229,P<0.001;t=20.955,P<0.001).After continuous intraperitoneal injection of anti-CD41 Ab for 2 days,the PLT between group II and group IV was statistically significant(t=7.493,P<0.001).On the 8 th day of the experiment,the PLT count between group II and group V was statistically different(t=10.829,P<0.001).In group III,MPV values decreased and PCT values increased on the 5 th day of the nAgB injection.On the 9 th day of the experience,compared with the blank control group,MPV values and the PCT values of the two groups were statistically significant(t=3.249,P<0.05;t=4.634,P<0.01).After ITP modeling was successful in group II,compared with group I,MPV and PCT were significantly different(t=9.615,P<0.001;t=28.698,P<0.001).After intraperitoneal injection of anti-CD41 Ab for 2 days,ITP modeling was successfully built.MPV and PCT values of group IV were significantly different from those of group II(t=5.794,P<0.001;t=6.831,P<0.001),and there was no statistically significant difference in PDW values(t=0.883,P>0.05).In group IV,after 2 days of nAgB injection,MPV and PCT values were significantly different from group II(t=6.971,P<0.001;t=5.507,P<0.01).There was no significant difference in PDW values between the two groups(t=1.686,P>0.05).Conclusion Immune injury induced ITP could be prevented and cured by AgB.